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In efforts to define pathways responsible for the development of neurofibromas and other cancers, transcriptomic and proteomic changes are evaluated in a non\u2010malignant <jats:italic>NF1<\/jats:italic> null cell line. <jats:italic>NF1<\/jats:italic> null HEK293 cells were created using CRISPR\/Cas9 technology and they are compared to parental cells that express neurofibromin. A total of 1222 genes and 132 proteins are found to be differentially expressed. The analysis is integrated to identify eight transcripts\/proteins that are differentially regulated in both analyses. Metacore Pathway analysis identifies Neurogenesis NGF\/TrkA MAPK\u2010mediated signaling alterations. Next, the data set is compared with other published studies that involve analysis of cells or tumors deficient for <jats:italic>NF1<\/jats:italic> and it is found that 141 genes recur in the sample and others; only thirteen of these genes recur in two or more studies. Genes\/proteins of interest are validated via q\u2010RT\u2010PCR or Western blot. It is shown that KRT8 and 14\u20103\u20103\u03c3\u00a0protein levels respond to exogenously introduced <jats:italic>mNf1<\/jats:italic> cDNA. Hence, transcripts\/proteins that respond to neurofibromin levels are identified and they can potentially be used as biomarkers.<\/jats:p>","DOI":"10.1002\/pmic.201800334","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T14:37:12Z","timestamp":1553524632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multi\u2010Omics Profiling for NF1 Target Discovery in Neurofibromin (NF1) Deficient Cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Rachel M.","family":"Carnes","sequence":"first","affiliation":[{"name":"Department of Genetics University of Alabama at Birmingham  35294 Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Mobley","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Alabama at Birmingham  35294 Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David K.","family":"Crossman","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Alabama at Birmingham  35294 Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Alabama at Birmingham  35294 Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce R.","family":"Korf","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Alabama at Birmingham  35294 Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Kesterson","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Alabama at Birmingham  35294 Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deeann","family":"Wallis","sequence":"additional","affiliation":[{"name":"Department of Genetics University of Alabama at Birmingham  35294 Birmingham AL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.464"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40246-017-0109-3"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2008.08.022"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/138945007780830773"},{"key":"e_1_2_7_4_3","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1182\/blood.V92.1.267.413a31_267_272","volume":"92","author":"Side L. 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