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As a result, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal\/lysosomal system. This abnormal accumulation results in a cascade of pathophysiological events including progressive, cerebellar neurodegeneration, among others. While significant progress has been made to better understand NPC1, the downstream effects of cholesterol storage and the major mechanisms that drive neurodegeneration remain unclear. In the current study, a) the use of a commercial, highly efficient standard flow\u2010ESI platform for protein biomarker identification is implemented and b) protein biomarkers are identified and evaluated at a terminal time point in the NPC1 null mouse model. In this study, alterations are observed in proteins related to fatty acid homeostasis, calcium binding and regulation, lysosomal regulation, and inositol biosynthesis and metabolism, as well as signaling by Rho family GTPases. New observations from this study include altered expression of Pcp2 and Limp2 in Npc1 mutant mice relative to control, with Pcp2 exhibiting multiple isoforms and specific to the cerebella. This study provides valuable insight into pathways altered in the late\u2010stage pathophysiology of NPC1.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201800432","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T22:13:27Z","timestamp":1553033607000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Quantitative, Label\u2010Free Proteomics in the Symptomatic Niemann\u2013Pick, Type C1 Mouse Model Using Standard Flow Liquid Chromatography and Thermal Focusing Electrospray Ionization"],"prefix":"10.1002","volume":"19","author":[{"given":"Melissa R.","family":"Pergande","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Illinois at Chicago  Chicago IL 60607 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thu T. 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