{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:23:40Z","timestamp":1790501020089,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,18]],"date-time":"2019-12-18T00:00:00Z","timestamp":1576627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"publisher","award":["NMRC\u2010NIG (R\u2010221\u2010000\u2010073\u2010511)"],"award-info":[{"award-number":["NMRC\u2010NIG (R\u2010221\u2010000\u2010073\u2010511)"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Candida albicans<\/jats:italic> is a major fungal pathogen, accounting for approximately 15% of healthcare infections with associated mortality as high as 40% in the case of systemic candidiasis. Antifungal agents for <jats:italic>C. albicans<\/jats:italic> infections are limited, and rising resistance is an inevitable problem. Therefore, understanding the mechanism behind antifungal responses is among the top research focuses in combating <jats:italic>Candida<\/jats:italic> infections. Herein, the recently developed <jats:italic>C. albicans<\/jats:italic> haploid model is employed to examine the association between mitochondrial fission, regulated by Dnm1, and the pathogen's response to antifungals. Proteomic analysis of <jats:italic>dnm1\u0394<\/jats:italic> and its wild\u2010type haploid parent, GZY803, reveal changes in proteins associated with mitochondrial structures and functions, cell wall, and plasma membrane. Antifungal susceptibility testing revealed that <jats:italic>dnm1\u0394<\/jats:italic> is more susceptible to SM21, a novel antifungal, than GZY803. Analyses of reactive oxygen species release, antioxidant response, lipid peroxidation, and membrane damages uncover an association between <jats:italic>dnm1\u0394<\/jats:italic> and the susceptibility to SM21. Dynasore\u2010induced mitochondrial inhibition in SC5314 diploids corroborate the findings. Interestingly, Dynasore\u2010primed SC5314 cultures exhibit increased susceptibility to all antifungals tested. These data suggest an important contribution of mitochondrial fission in antifungal susceptibility of <jats:italic>C. albicans<\/jats:italic>. Hence, mitochondrial fission can be a potential target for combined therapy in anti\u2010<jats:italic>C. albicans<\/jats:italic> treatment.<\/jats:p>","DOI":"10.1002\/pmic.201900240","type":"journal-article","created":{"date-parts":[[2019,12,7]],"date-time":"2019-12-07T05:15:50Z","timestamp":1575695750000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Proteomics Analysis of <i>Candida albicans dnm1<\/i> Haploid Mutant Unraveled the Association between Mitochondrial Fission and Antifungal Susceptibility"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4172-3670","authenticated-orcid":false,"given":"Thuyen","family":"Truong","sequence":"first","affiliation":[{"name":"Oral Sciences, Faculty of Dentistry National University of Singapore  9 Lower Kent Ridge Road Singapore 119085"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guisheng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology Agency for Science, Technology and Research  61 Biopolis Drive, Proteos Singapore 138673"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teck Kwang","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Faculty of Science, National University of Singapore  16 Science Drive 4, S2 Singapore 117558"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Cao","sequence":"additional","affiliation":[{"name":"Oral Sciences, Faculty of Dentistry National University of Singapore  9 Lower Kent Ridge Road Singapore 119085"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9001-9068","authenticated-orcid":false,"given":"Li Mei","family":"Pang","sequence":"additional","affiliation":[{"name":"National Dental Research Institute Singapore Singhealth Duke NUS, Singapore  5 Second Hospital Ave Singapore 168938"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yew Mun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Faculty of Science, National University of Singapore  16 Science Drive 4, S2 Singapore 117558"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingsong","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Faculty of Science, National University of Singapore  16 Science Drive 4, S2 Singapore 117558"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cell Biology Agency for Science, Technology and Research  61 Biopolis Drive, Proteos Singapore 138673"},{"name":"Department of Biochemistry, Yong Loo Lin School of Medicine National University of Singapore  10 Medical Dr Singapore 117597"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaminda Jayampath","family":"Seneviratne","sequence":"additional","affiliation":[{"name":"National Dental Research Institute Singapore Singhealth Duke NUS, Singapore  5 Second Hospital Ave Singapore 168938"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,12,18]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-011-1064-7"},{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.22913"},{"key":"e_1_2_8_1_3","doi-asserted-by":"publisher","DOI":"10.1086\/421946"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/713687"},{"key":"e_1_2_8_2_2","first-page":"2173","volume":"7","author":"Whaley S. G.","year":"2016","journal-title":"Front. Microbiol."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.05184-11"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00182-07"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07503.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1199"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000753"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11865"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12433"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M116.061523"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1219855"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.09.003"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1708"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/13014"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071939"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.2.333"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600422"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085836"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.04.002"},{"key":"e_1_2_8_18_1","unstructured":"National Committee for Clinical Laboratory Standards.Reference method for broth dilution antifungal susceptibility testing of filamentous fungi. Approved standard. NCCLS document M38\u2010A. National Committee for Clinical Laboratory Standards Wayne PA2002."},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dib.2015.10.016"},{"key":"e_1_2_8_19_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/978-1-60761-157-8_12","volume-title":"Proteomics: Methods and Protocols","author":"Schmidt C.","year":"2009"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.023085"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.25.14863"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti551"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00866.x"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0376-x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fov027"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-101011-155756"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.38.s1.79.86"},{"key":"e_1_2_8_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3193-3198.1999"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr060474i"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)61523-1"},{"key":"e_1_2_8_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.01.005"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1426"},{"key":"e_1_2_8_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom5010142"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601945103"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00048-2"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.01.023"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2004.12.010"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.12.5398-5402.1999"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2010.00663.x"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300855110"},{"key":"e_1_2_8_36_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.073080"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01520-12"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00349-06"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01271-10"},{"key":"e_1_2_8_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrp.2012.04.001"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780310001616528"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1188235"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2013.03.023"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201900240","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201900240","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pmic.201900240","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201900240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:37:56Z","timestamp":1761057476000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201900240"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,18]]},"references-count":55,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1002\/pmic.201900240"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201900240","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,18]]},"assertion":[{"value":"2019-07-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1900240"}}