{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T13:04:48Z","timestamp":1778591088838,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"15-16","license":[{"start":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T00:00:00Z","timestamp":1626220800000},"content-version":"am","delay-in-days":350,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T00:00:00Z","timestamp":1595980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R15CA164929"],"award-info":[{"award-number":["R15CA164929"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CHE\u20101228656"],"award-info":[{"award-number":["CHE\u20101228656"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study identifies the main changes in protein expression in human breast tumors compared to normal breast tissue. Malignant tumors (32) and normal breast tissue samples (23), from formaldehyde\u2010fixed, paraffin\u2010embedded specimens are subjected to discovery proteomics using liquid chromatography\/tandem mass spectrometry, with spectral counts for quantitation. The dataset contains 1406 proteins. Differential expression is measured using a method that takes advantage of estimates of the percentage of tumor on a slide. This analysis shows that the major classes of proteins over\u2010expressed by tumors are RNA\u2010binding, heat shock and DNA repair proteins. RNA\u2010binding proteins, including heterogeneous nuclear ribonucleoproteins (HNRNPs), SR splice factors (SRSF) and elongation factors form the largest group. Comparison with results from another study demonstrates that the RNA\u2010binding proteins are associated specifically with malignant transformation, rather than with cell proliferation. HNRNP and SRSF proteins help define splice sites in normal cells. Their over\u2010expression may dysregulate splicing, which in turn has the potential to promote malignant transformation.<\/jats:p>","DOI":"10.1002\/pmic.202000044","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T13:49:11Z","timestamp":1594734551000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Over\u2010Expression of RNA Processing, Heat Shock, and DNA Repair Proteins in Breast Tumor Compared to Normal Tissue"],"prefix":"10.1002","volume":"20","author":[{"given":"Ten\u2010Yang","family":"Yen","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry San Francisco State University  1600 Holloway Ave. San Francisco CA 94132 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Pathology University of California  San Diego CA 92103 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald","family":"Pizzo","sequence":"additional","affiliation":[{"name":"Department of Pathology University of California  San Diego CA 92103 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moe","family":"Thein","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry San Francisco State University  1600 Holloway Ave. San Francisco CA 94132 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce A.","family":"Macher","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry San Francisco State University  1600 Holloway Ave. San Francisco CA 94132 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2398-4765","authenticated-orcid":false,"given":"Leslie C.","family":"Timpe","sequence":"additional","affiliation":[{"name":"Department of Biology San Francisco State University  1600 Holloway Ave. San Francisco CA 94132 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,29]]},"reference":[{"key":"e_1_2_5_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18003"},{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014-018-9202-4"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr2007736"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201100044"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-3711"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.241968.114"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-18-0068"},{"key":"e_1_2_5_8_1","first-page":"1","volume-title":"RNA Binding Proteins","author":"Michelle L.","year":"2012"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjv027"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.51"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1178"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-13-0253"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-015-0168-9"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.09.019"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2015.00142"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.10.071"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.202000044","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202000044","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pmic.202000044","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/pmic.202000044","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202000044","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T11:01:13Z","timestamp":1761044473000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.202000044"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,29]]},"references-count":16,"journal-issue":{"issue":"15-16","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["10.1002\/pmic.202000044"],"URL":"https:\/\/doi.org\/10.1002\/pmic.202000044","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,29]]},"assertion":[{"value":"2020-02-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2000044"}}