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Mass spectrometry\u2010based proteomic analysis of Triton X\u2010114 fractions of proteomic analysis of <jats:italic>Leptospira interrogans<\/jats:italic> identified 2957 unique proteins distributed across the fractions. The results are compared with bioinformatics predictions on their subcellular localization and pathogenic nature. Analysis of the distribution of proteins across the Triton X\u2010114 fractions with the predicted characteristics is performed based on \u201cnumber\u201d of unique type of proteins, and \u201cquantity\u201d which represents the amount of unique protein. The highest number of predicted outer membrane proteins (OMPs) and pathogenic proteins are found in aqueous and pellet fractions, whereas detergent fraction representing the OM has the highest quantity of OMPs and pathogenic proteins though lower in number than the aqueous and pellet fractions. This leaves the possibility of an upsurge in pathogenic proteins and OMPs on the OM under pathogenic conditions suggesting their potential use to combat leptospirosis. Further, the Triton X\u2010114 subcellular fractions are more correlated to enrichment of pathogenic proteins predicted by MP3 software than predicted localization.<\/jats:p>","DOI":"10.1002\/pmic.202000170","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T14:08:26Z","timestamp":1599055706000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Triton X\u2010114 Fractionated Subcellular Proteome of <i>Leptospira interrogans<\/i> Shows Selective Enrichment of Pathogenic and Outer Membrane Proteins in the Detergent Fraction"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9146-4887","authenticated-orcid":false,"given":"Sikha","family":"Thoduvayil","sequence":"first","affiliation":[{"name":"Indian Council of Medical Research Regional Medical Research Centre Port Blair  Dollygunj Port Blair 744103 India"},{"name":"Department of Pathology Jawaharlal Institute of Postgraduate Medical Education and Research  Puducherry 605006 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunasekaran","family":"Dhandapani","sequence":"additional","affiliation":[{"name":"Indian Council of Medical Research Regional Medical Research Centre Port Blair  Dollygunj Port Blair 744103 India"},{"name":"Department of Chemical Sciences Ariel University  Ariel 70400 Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1802-3430","authenticated-orcid":false,"given":"Rahul","family":"Brahma","sequence":"additional","affiliation":[{"name":"Indian Council of Medical Research Regional Medical Research Centre Port Blair  Dollygunj Port Blair 744103 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9556-3150","authenticated-orcid":false,"given":"Rex","family":"Devasahayam Arokia Balaya","sequence":"additional","affiliation":[{"name":"Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangaluru 575018 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiran K.","family":"Mangalaparthi","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bengaluru 560066 India"},{"name":"NIMHANS\u2010IOB Proteomics and Bioinformatics Laboratory Neurobiology Research Centre National Institute of Mental Health and Neurosciences  Bengaluru 560029 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishna","family":"Patel","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bengaluru 560066 India"},{"name":"Amrita School of Biotechnology Amrita Vishwa Vidyapeetham  Kollam 690525 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manish","family":"Kumar","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bengaluru 560066 India"},{"name":"Manipal Academy of Higher Education  Manipal 576104 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5771-2189","authenticated-orcid":false,"given":"Jebasingh","family":"Tennyson","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Madurai Kamaraj University  Madurai 625021 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2031-9049","authenticated-orcid":false,"given":"P. 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