{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:14:44Z","timestamp":1791274484383,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sporozoites of the malaria parasite\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    are transmitted by mosquitoes and infect the liver for an initial and obligatory round of replication, before exponential multiplication in the blood and onset of the disease. Sporozoites and liver stages provide attractive targets for malaria vaccines and prophylactic drugs. In this context, defining the parasite proteome is important to explore the parasite biology and to identify potential targets for antimalarial strategies. Previous studies have determined the total proteome of sporozoites from the two main human malaria parasites,\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    and\n                    <jats:italic>P. vivax<\/jats:italic>\n                    , as well as\n                    <jats:italic>P. yoelii<\/jats:italic>\n                    , which infects rodents. Another murine malaria parasite,\n                    <jats:italic>P. berghei<\/jats:italic>\n                    , is widely used to investigate the parasite biology. However, a deep view of the proteome of\n                    <jats:italic>P. berghei<\/jats:italic>\n                    sporozoites is still missing. To fill this gap, we took advantage of the highly sensitive timsTOF PRO mass spectrometer, combined with three alternative methods for sporozoite purification, to identify the proteome of\n                    <jats:italic>P. berghei<\/jats:italic>\n                    sporozoites using low numbers of parasites. This study provides a reference proteome for\n                    <jats:italic>P. berghei<\/jats:italic>\n                    sporozoites, identifying a core set of proteins expressed across species, and illustrates how the unprecedented sensitivity of the timsTOF PRO system enables deep proteomic analysis from limited sample amounts.\n                  <\/jats:p>","DOI":"10.1002\/pmic.202000305","type":"journal-article","created":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T05:36:35Z","timestamp":1610775395000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["In\u2010depth proteomic analysis of\n                    <i>Plasmodium berghei<\/i>\n                    sporozoites using trapped ion mobility spectrometry with parallel accumulation\u2010serial fragmentation"],"prefix":"10.1002","volume":"21","author":[{"given":"Soumia","family":"Hamada","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, UMS PASS, Plateforme Post\u2010g\u00e9nomique de la Piti\u00e9 Salp\u00eatri\u00e8re (P3S)  Paris France"},{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI\u2010Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9dric","family":"Pionneau","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, UMS PASS, Plateforme Post\u2010g\u00e9nomique de la Piti\u00e9 Salp\u00eatri\u00e8re (P3S)  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Parizot","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Centre d'Immunologie et des Maladies Infectieuses, CIMI\u2010Paris, Assistance Publique H\u00f4pitaux de Paris, H\u00f4pital Piti\u00e9\u2010Salp\u00eatri\u00e8re  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Silvie","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI\u2010Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solenne","family":"Chardonnet","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, UMS PASS, Plateforme Post\u2010g\u00e9nomique de la Piti\u00e9 Salp\u00eatri\u00e8re (P3S)  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8667-2208","authenticated-orcid":false,"given":"Carine","family":"Marinach","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI\u2010Paris  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,23]]},"reference":[{"key":"e_1_2_12_2_1","unstructured":"World Health Organization(2019). 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