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Herein, we report on the proof\u2010of\u2010concept of a simple method enabling in vitro detection of a structural spike protein subunit from the SARS\u2010CoV\u20102 (S<jats:sub>1<\/jats:sub>) in aqueous samples. At the core of this discovery lies the well\u2010known paradigm of monitoring the capacitive current across a reconstituted zwitterionic lipid membrane subjected to a periodic transmembrane potential, followed by the real\u2010time spectral analysis enabling the extraction of the second harmonic of the capacitive current. Subsequent changes in the amplitude of this harmonic recorded during lipid membrane\u2013S<jats:sub>1<\/jats:sub> interactions were correlated with alterations induced in the inner membrane potential profile by the S<jats:sub>1<\/jats:sub> protein subunit adsorption, and were shown to be augmented by ionic strength, the presence of a specific monoclonal antibody designed against the S<jats:sub>1<\/jats:sub> subunit and the angiotensin\u2010converting enzyme 2 (ACE2) protein receptor, and uninhibited by the presence of other human serum proteins.<\/jats:p>","DOI":"10.1002\/pmic.202100047","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T13:56:50Z","timestamp":1632923810000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Teaching an old dog new tricks: A lipid membrane\u2010based electric immunosensor for real\u2010time probing of the spike S<sub>1<\/sub> protein subunit from SARS\u2010CoV\u20102"],"prefix":"10.1002","volume":"22","author":[{"given":"Alina","family":"Asandei","sequence":"first","affiliation":[{"name":"Sciences Department Interdisciplinary Research Institute Alexandru I. 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Cuza University  Iasi Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoonkyung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science and Research Center for Proteinaceous Materials (RCPM) Chosun University  Gwangju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9388-7266","authenticated-orcid":false,"given":"Tudor","family":"Luchian","sequence":"additional","affiliation":[{"name":"Department of Physics Alexandru I. 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