{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:31:08Z","timestamp":1790771468154,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T00:00:00Z","timestamp":1635811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008322","name":"CSL Behring","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fibroblasts (Fb) are key effector cells in systemic sclerosis (SSc). Fb stimulation with transforming growth factor beta 1 (TGF\u2010\u03b21) is considered as a positive control in studies assessing fibrogenesis. The lack of standardization of TGF\u2010\u03b21 stimulation might be responsible for discrepancies in experiments performed in different conditions. Using quantitative proteomics analysis, we evaluated the impact of changes in experimental conditions on proteomic profiles of primary Fb. Principal component analysis (PCA) identified several groups of differentially expressed proteins influenced by cell passage, culture medium, and both concentration and duration of exposure to TGF\u2010\u03b21 stimulation. Bioinformatics analysis revealed that late passages expressed proteins involved in senescence. TGF\u2010\u03b21 concentration and time of stimulation were correlated with the expression of proteins involved in the fibrogenesis and inflammatory processes. These data underline the need for standardization of culture conditions to allow inter\u2010data comparisons in future in vitro studies, especially when using \u201comics\u201d approaches.<\/jats:p>","DOI":"10.1002\/pmic.202100116","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T17:32:56Z","timestamp":1634751176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Passages in culture and stimulation conditions influence protein expression of primary fibroblasts"],"prefix":"10.1002","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7640-9465","authenticated-orcid":false,"given":"Solange","family":"Vivier","sequence":"first","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8370-5983","authenticated-orcid":false,"given":"Aur\u00e9lien","family":"Chepy","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"},{"name":"CHU Lille D\u00e9partement de M\u00e9decine Interne et Immunologie Clinique Centre de R\u00e9f\u00e9rence des Maladies Auto\u2010immunes Syst\u00e9miques Rares du Nord et Nord\u2010Ouest de France (CeRAINO)  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4723-8206","authenticated-orcid":false,"given":"Fabrice","family":"Bray","sequence":"additional","affiliation":[{"name":"Univ. Lille CNRS USR 3290 \u2010 MSAP \u2010 Miniaturisation pour la Synth\u00e8se, l'Analyse et la Prot\u00e9omique  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8232-9405","authenticated-orcid":false,"given":"Thomas","family":"Guerrier","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"},{"name":"Institut d'Immunologie Centre de Biologie Pathologie CHU Lille  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8494-3329","authenticated-orcid":false,"given":"Silvia","family":"Speca","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0135-675X","authenticated-orcid":false,"given":"St\u00e9phanie","family":"Flament","sequence":"additional","affiliation":[{"name":"Univ. Lille CNRS USR 3290 \u2010 MSAP \u2010 Miniaturisation pour la Synth\u00e8se, l'Analyse et la Prot\u00e9omique  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1047-1274","authenticated-orcid":false,"given":"Manel","family":"Jendoubi","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u00eft\u00e9","family":"Balden","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"},{"name":"Institut d'Immunologie Centre de Biologie Pathologie CHU Lille  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3266-8860","authenticated-orcid":false,"given":"Christian","family":"Rolando","sequence":"additional","affiliation":[{"name":"Univ. Lille CNRS USR 3290 \u2010 MSAP \u2010 Miniaturisation pour la Synth\u00e8se, l'Analyse et la Prot\u00e9omique  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7432-847X","authenticated-orcid":false,"given":"Eric","family":"Hachulla","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"},{"name":"CHU Lille D\u00e9partement de M\u00e9decine Interne et Immunologie Clinique Centre de R\u00e9f\u00e9rence des Maladies Auto\u2010immunes Syst\u00e9miques Rares du Nord et Nord\u2010Ouest de France (CeRAINO)  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1840-1817","authenticated-orcid":false,"given":"David","family":"Launay","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"},{"name":"CHU Lille D\u00e9partement de M\u00e9decine Interne et Immunologie Clinique Centre de R\u00e9f\u00e9rence des Maladies Auto\u2010immunes Syst\u00e9miques Rares du Nord et Nord\u2010Ouest de France (CeRAINO)  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8962-3719","authenticated-orcid":false,"given":"Sylvain","family":"Dubucquoi","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"},{"name":"Institut d'Immunologie Centre de Biologie Pathologie CHU Lille  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3083-2441","authenticated-orcid":false,"given":"Vincent","family":"Sobanski","sequence":"additional","affiliation":[{"name":"Univ. Lille Inserm CHU Lille U1286 \u2010 INFINITE \u2010 Institute for Translational Research in Inflammation  Lille France"},{"name":"CHU Lille D\u00e9partement de M\u00e9decine Interne et Immunologie Clinique Centre de R\u00e9f\u00e9rence des Maladies Auto\u2010immunes Syst\u00e9miques Rares du Nord et Nord\u2010Ouest de France (CeRAINO)  Lille France"},{"name":"Institut Universitaire de France (IUF)  France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1300575"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)30933-9"},{"issue":"2","key":"e_1_2_9_4_1","first-page":"69","article-title":"The mighty fibroblast and its utility in scleroderma research","volume":"2","author":"Garret S. M.","year":"2017","journal-title":"Journal of Scleroderma Related Disorders"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2019.12.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i28.3635"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-018-9800-z"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.69"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp050297a"},{"issue":"1","key":"e_1_2_9_10_1","first-page":"70","article-title":"Effect of passage number and culture time on the expression and activity of insulin\u2010degrading enzyme in Caco\u20102 cells","volume":"22","author":"Farsani T. M.","year":"2018","journal-title":"Iranian Biomedical Journal"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-214865"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-08247-x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12946"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr4010019"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.7b00069"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr101065j"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.031591"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3901"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1055"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1038"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202100116","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pmic.202100116","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202100116","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:53:40Z","timestamp":1761040420000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.202100116"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,2]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/pmic.202100116"],"URL":"https:\/\/doi.org\/10.1002\/pmic.202100116","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,2]]},"assertion":[{"value":"2021-04-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2100116"}}