{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T20:04:26Z","timestamp":1761163466506,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2021,10,23]],"date-time":"2021-10-23T00:00:00Z","timestamp":1634947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The existing protein annotation in chicken is mostly limited to computational predictions based on orthology to other proteins, which often leads to a significant underestimation of the function of these proteins. Genome\u2010scale experimental annotation can provide insight into the actual enzymatic activities of chicken proteins. Amongst post\u2010translational modifications, ubiquitination is of interest as anomalies in ubiquitination are implicated in such diseases as inflammatory disorders, infectious diseases, or malignancies. Ubiquitination is controlled by deubiquitinases (DUBs), which remove ubiquitin from protein substrates. However, the DUBs have not been systematically annotated and quantified in chicken tissues. Here we used a chemoproteomics approach, which is based on active\u2010site probes specific to DUBs, and identified 26 active DUBs in the chicken spleen, cecum, and liver.<\/jats:p>","DOI":"10.1002\/pmic.202100122","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T17:13:57Z","timestamp":1634145237000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of active deubiquitinases in the chicken tissues"],"prefix":"10.1002","volume":"22","author":[{"given":"Bindu","family":"Nanduri","sequence":"first","affiliation":[{"name":"Department of Comparative Biomedical Sciences College of Veterinary Medicine Mississippi State University  Mississippi State Mississippi USA"},{"name":"Institute for Genomics Biocomputing and Biotechnology Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathy R.","family":"Gresham","sequence":"additional","affiliation":[{"name":"Institute for Genomics Biocomputing and Biotechnology Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Science College of Agricultural and Life Sciences University of Florida  Gainesville USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard H.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Population Medicine College of Veterinary Medicine Mississippi State University  Mississippi State Mississippi USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0459-7716","authenticated-orcid":false,"given":"Mariola J.","family":"Edelmann","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell 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