{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:35:15Z","timestamp":1790476515433,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004189","name":"Max-Planck-Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004410","name":"European Molecular Biology Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A major challenge in managing depression is that antidepressant drugs take a long time to exert their therapeutic effects. For the development of faster\u2010acting treatments, it is crucial to get an improved understanding of the molecular mechanisms underlying antidepressant mode of action. Here, we used a novel mass spectrometry\u2010based workflow to investigate how antidepressant treatment affects brain protein turnover at single\u2010cell and subcellular resolution. We combined stable isotope metabolic labeling, quantitative Tandem Mass Spectrometry (qTMS) and Multi\u2010isotope Imaging Mass Spectrometry (MIMS) to simultaneously quantify and image protein synthesis and turnover in hippocampi of mice treated with the antidepressant paroxetine. We identified changes in turnover of individual hippocampal proteins that reveal altered metabolism\u2010mitochondrial processes and report on subregion\u2010specific turnover patterns upon paroxetine treatment. This workflow can be used to investigate brain protein turnover changes in vivo upon pharmacological interventions at a resolution and precision that has not been possible with other methods to date. Our results reveal acute paroxetine effects on brain protein turnover and shed light on antidepressant mode of action.<\/jats:p>","DOI":"10.1002\/pmic.202100244","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T03:43:33Z","timestamp":1648698213000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Antidepressant treatment effects on hippocampal protein turnover: Molecular and spatial insights from mass spectrometry"],"prefix":"10.1002","volume":"22","author":[{"given":"Christoph W.","family":"Turck","sequence":"first","affiliation":[{"name":"Max Planck Institute of Psychiatry  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Webhofer","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Psychiatry  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Reckow","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Psychiatry  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Moy","sequence":"additional","affiliation":[{"name":"Division of Genetics Brigham and Women's Hospital and Harvard Medical School  Boston Massachusetts USA"},{"name":"National Resource for Imaging Mass Spectrometry (NRIMS)  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Genetics Brigham and Women's Hospital and Harvard Medical School  Boston Massachusetts USA"},{"name":"National Resource for Imaging Mass Spectrometry (NRIMS)  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Guillermier","sequence":"additional","affiliation":[{"name":"Division of Genetics Brigham and Women's Hospital and Harvard Medical School  Boston Massachusetts USA"},{"name":"National Resource for Imaging Mass Spectrometry (NRIMS)  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Collin","family":"Poczatek","sequence":"additional","affiliation":[{"name":"Division of Genetics Brigham and Women's Hospital and Harvard Medical School  Boston Massachusetts USA"},{"name":"National Resource for Imaging Mass Spectrometry (NRIMS)  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela D.","family":"Filiou","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Psychiatry  Munich Germany"},{"name":"Department of Biological Applications and Technology School of Health Sciences University of Ioannina  Ioannina Greece"},{"name":"Foundation for Research and Technology\u2010Hellas (FORTH) Biomedical Research Institute  Ioannina 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