{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T12:36:14Z","timestamp":1792499774855,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T00:00:00Z","timestamp":1674691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Urinary glycoproteins associated with aggressive prostate cancer (AG\u2010PCa) were previously reported using post\u2010digital rectal examination (DRE) urine specimens. To explore the potential of using pre\u2010DRE urine specimens for detecting AG\u2010PCa, we compared glycoproteins between pre\u2010 and post\u2010DRE urine specimens, verified the previously identified post\u2010DRE AG\u2010PCa\u2010associated urinary glycoproteins in pre\u2010DRE urine specimens, and explored potential new glycoproteins for AG\u2010PCa detection in pre\u2010DRE urine specimens. Quantitative glycoproteomic data were acquired for 154 pre\u2010DRE urine specimens from 41 patients with no cancer at biopsy, 48 patients with non\u2010AG\u2010PCa (Gleason score\u00a0=\u00a06), and 65 patients with AG\u2010PCa (Gleason score 7 or above). Compared to glycopeptides from the post\u2010DRE urine data, humoral immunity\u2010related proteins were enriched in pre\u2010DRE urine samples, whereas cell mediated immune response proteins were enriched in post\u2010DRE urine samples. Analyses of AG\u2010PCa\u2010associated glycoproteins from pre\u2010DRE urine revealed that the three urinary glycoproteins, prostate\u2010specific antigen (PSA), prostatic acid phosphatase (ACPP), and CD97 antigen (CD97) that were previously identified in post\u2010DRE urine samples, were also observed as AG\u2010PCa associated glycoproteins in pre\u2010DRE urine. In addition, we identified three new glycoproteins, fibrillin 1 (FBN1), vitronectin (VTN), and hemicentin 2 (HMCN2), to be potentially associated with AG\u2010PCa in pre\u2010DRE urine specimens. In summary, glycoprotein profiles differ between pre\u2010 and post\u2010DRE urine specimens. The identified AG\u2010PCa\u2010associated glycoproteins may be further evaluated in large cohort of pre\u2010DRE urine specimens for detecting clinically significant PCa.<\/jats:p>","DOI":"10.1002\/pmic.202200023","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T10:56:21Z","timestamp":1670496981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Differentially expressed glycoproteins in pre\u2010 and post\u2010digital rectal examination urine samples for detecting aggressive prostate cancer"],"prefix":"10.1002","volume":"23","author":[{"given":"Yuefan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tung\u2010Shing Mamie","family":"Lih","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins 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