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Its molecular subtypes are based on the presence\/absence of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). MACL\u20101 and MGSO\u20103 are cell lines derived from primary tumor sites of patients diagnosed with luminal A subtype carcinoma (ER+\/PR+\/HER2\u2212) and ductal carcinoma in situ (ER\u2212\/PR\u2212\/HER2+), respectively. However, these cell lines lost the expression of these markers over cell culturing, and both have triple\u2010negative phenotypes (ER\u2212\/PR\u2212\/HER2\u2212), which has the poorest prognosis. Here, we sought to study the proteome signature of MGSO\u20103 and MACL\u20101, comparing them with the epithelial cell line MCF\u201010A and the well\u2010established metastatic\u2010derived breast cancer cell line MDA\u2010MB\u2010231. Our results showed that proteins associated with the tricarboxylic acid cycle (TCA) and oxidative phosphorylation (OXPHOS) were upregulated in MGSO\u20103 and MACL\u20101 cells. These cell lines also showed upregulation of pro\u2010apoptotic proteins when compared with MDA\u2010MB\u2010231. The molecular differences highlighted in this study may clarify the molecular basis behind cancer cells functioning and may reveal novel signatures across the breast cancer cell models.<\/jats:p>","DOI":"10.1002\/pmic.202200095","type":"journal-article","created":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T14:59:56Z","timestamp":1654354796000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Proteomic analysis reveals stage\u2010specific reprogramed metabolism for the primary breast cancer cell lines MGSO\u20103 and MACL\u20101"],"prefix":"10.1002","volume":"22","author":[{"given":"Fabio Henrique Guimar\u00e3es","family":"Braga","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics Institute of Biological Sciences Federal University of Minas Gerais  Belo Horizonte MG Brazil"},{"name":"Department of Biochemistry and Immunology Institute of Biological Sciences Federal University of 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