{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:38:05Z","timestamp":1761093485113,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T00:00:00Z","timestamp":1672704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The innate immune protection provided by cationic antimicrobial peptides (CAMPs) has been shown to extend to antiviral activity, with putative mechanisms of action including direct interaction with host cells or pathogen membranes. The lack of therapeutics available for the treatment of viruses such as Venezuelan equine encephalitis virus (VEEV) underscores the urgency of novel strategies for antiviral discovery. American alligator plasma has been shown to exhibit strong in vitro antibacterial activity, and functionalized hydrogel particles have been successfully employed for the identification of specific CAMPs from alligator plasma. Here, a novel bait strategy in which particles were encapsulated in membranes from either healthy or VEEV\u2010infected cells was implemented to identify peptides preferentially targeting infected cells for subsequent evaluation of antiviral activity. Statistical analysis of peptide identification results was used to select five candidate peptides for testing, of which one exhibited a dose\u2010dependent inhibition of VEEV and also significantly inhibited infectious titers. Results suggest our bioprospecting strategy provides a versatile platform that may be adapted for antiviral peptide identification from complex biological samples.<\/jats:p>","DOI":"10.1002\/pmic.202200237","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T11:05:22Z","timestamp":1670497522000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bioprospecting the American Alligator Peptidome for antiviral peptides against Venezuelan equine encephalitis virus"],"prefix":"10.1002","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8039-4358","authenticated-orcid":false,"given":"Amy","family":"Carfagno","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih\u2010Chao","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Systems Biology George Mason University  Manassas Virginia USA"},{"name":"National Taiwan Ocean University  Keelung City Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liana","family":"Chafran","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Akhrymuk","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology Virginia Polytechnic Institute and State University  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria","family":"Callahan","sequence":"additional","affiliation":[{"name":"School of Systems Biology George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marynet","family":"Po","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaling","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amaal","family":"Altalhi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P.","family":"Durkin","sequence":"additional","affiliation":[{"name":"Chemistry Department U.S. Naval Academy  Annapolis Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Russo","sequence":"additional","affiliation":[{"name":"Center for Applied Proteomics and Molecular Medicine George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kent A.","family":"Vliet","sequence":"additional","affiliation":[{"name":"Department of Biology University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bobbie\u2010Jo","family":"Webb\u2010Robertson","sequence":"additional","affiliation":[{"name":"Biological Sciences Division Pacific Northwest National Laboratory  Richland Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kylene","family":"Kehn\u2010Hall","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology Virginia Polytechnic Institute and State University  Blacksburg Virginia USA"},{"name":"Center for Emerging, Zoonotic, and Arthropod\u2010borne Pathogens Virginia Polytechnic Institute and State University  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barney","family":"Bishop","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry George Mason University  Manassas Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0117394"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2017.01.011"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u201011\u2010114"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2016.02.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104925200"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1021"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00904\u201015"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00764"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2022.2141224"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/v9100279"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2019.04.004"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00857"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9NR01292C"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191983"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3791\/52065"},{"key":"e_1_2_9_17_1","unstructured":"Bishop B.(2022).MassIVE MSV000089560 \u2010 Bioprospecting the American Alligator Peptidome for Antiviral Peptides Against Venezuelan Equine Encephalitis Virus [Data set]. MassIVE. ftp:\/\/massive.ucsd.edu\/MSV000089560."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr1005247"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2017.11.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551\u2010016\u20100218\u20106"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma9804951"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/la902423v"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm0507296"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm200266z"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2012.212"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep34422"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b04810"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1196"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.010587"},{"key":"e_1_2_9_30_1","unstructured":"PEAKS X [Computer software]. (2018).Waterloo Ontario Canada: Bioinformatics Solutions Inc."},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517\u20106161.1995.tb02031.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-9863-7_1215"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2019.02.002"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(05)80360\u20102"},{"volume-title":"Immunobiology: the immune system in health and disease","year":"2001","author":"Janeway C. A.","key":"e_1_2_9_35_1"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2018.08.004"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202200237","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pmic.202200237","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202200237","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T11:46:11Z","timestamp":1761047171000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.202200237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,3]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/pmic.202200237"],"URL":"https:\/\/doi.org\/10.1002\/pmic.202200237","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"type":"print","value":"1615-9853"},{"type":"electronic","value":"1615-9861"}],"subject":[],"published":{"date-parts":[[2023,1,3]]},"assertion":[{"value":"2022-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2200237"}}