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We aimed to investigate the changes in RPE\/choroid complex metabolism in a form deprivation myopia model after dopamine D2 receptor (D2R) modulation. Guinea pigs were randomly divided into normal (NC), form deprivation myopia (FDM), and FDM treated with dopamine D2R antagonist groups. Differential metabolites were screened using SIMCA\u2010P software and MetaboAnalyst metabolomics analysis tool. Functions of differential metabolites were analyzed using KEGG enrichment pathways. Relative to the NC group, 38 differential metabolites were identified, comprising 29 increased metabolites (including nicotinic acid, cytosine, and glutamate) and 9 decreased metabolites, of which proline exhibited the largest decrease. Pathway analysis revealed regulation of arginine\/proline and aspartate\/glutamate metabolism. Intravitreal D2R antagonist injection increased proline concentrations and activated arginine\/proline and purine metabolism pathways. In sum, D2R antagonists alleviated the myopia trend of refractive biological parameters in form deprivation myopic guinea pigs, suggesting the involvement of dopamine D2R signaling in myopia pathogenesis. The RPE\/choroid may provide glutamate to the retina by activating proline metabolism via metabolic coupling with the retina. Dopamine D2R antagonism may modulate proline\/arginine metabolic pathways in the RPE\/choroid and regulate metabolism, information presentation, and myopia.<\/jats:p>","DOI":"10.1002\/pmic.202200325","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:45:40Z","timestamp":1690332340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effects of dopamine D2 receptor antagonists on retinal pigment epithelial\/choroid complex metabolism in form\u2010deprived myopic guinea pigs"],"prefix":"10.1002","volume":"23","author":[{"given":"Pinghui","family":"Wei","sequence":"first","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual Science  Tianjin PR China"},{"name":"Nankai University Eye Institute Nankai University Affiliated Eye Hospital Nankai University  Tianjin PR China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0079-3023","authenticated-orcid":false,"given":"Guoge","family":"Han","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual Science  Tianjin PR China"},{"name":"Nankai University Eye Institute Nankai University Affiliated Eye Hospital Nankai University  Tianjin PR China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Visual Science  Tianjin PR China"},{"name":"Nankai University Eye Institute Nankai University Affiliated Eye Hospital Nankai University  Tianjin PR China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin PR 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