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It has been established that cachexia inducing cancer cells secrete proteins and extracellular vesicles (EVs) that can induce muscle atrophy. Though several studies examined these cancer\u2010cell derived factors, targeting some of these components have shown little or no clinical benefit. To develop new therapies, understanding of the dysregulated proteins and signaling pathways that regulate catabolic gene expression during muscle wasting is essential. Here, we sought to examine the effect of conditioned media (CM) that contain secreted factors and EVs from cachexia inducing C26 colon cancer cells on C2C12 myotubes using mass spectrometry\u2010based label\u2010free quantitative proteomics. We identified significant changes in the protein profile of C2C12 cells upon exposure to C26\u2010derived CM. Functional enrichment analysis revealed enrichment of proteins associated with inflammation, mitochondrial dysfunction, muscle catabolism, ROS production, and ER stress in CM treated myotubes. Furthermore, strong downregulation in muscle structural integrity and development and\/or regenerative pathways were observed. Together, these enriched proteins in atrophied muscle could be utilized as potential muscle wasting markers and the dysregulated biological processes could be employed for therapeutic benefit in cancer\u2010induced muscle wasting.<\/jats:p>","DOI":"10.1002\/pmic.202300020","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T06:55:11Z","timestamp":1698303311000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Proteomics analysis of C2C12 myotubes treated with atrophy inducing cancer cell\u2010derived factors"],"prefix":"10.1002","volume":"24","author":[{"given":"Akbar L.","family":"Marzan","sequence":"first","affiliation":[{"name":"Department of Biochemistry La Trobe Institute for Molecular Science La Trobe University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sai 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