{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:08:43Z","timestamp":1790564923370,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:00:00Z","timestamp":1695686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["322900939"],"award-info":[{"award-number":["322900939"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["445703531"],"award-info":[{"award-number":["445703531"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000921","name":"European Cooperation in Science and Technology","doi-asserted-by":"publisher","award":["21165"],"award-info":[{"award-number":["21165"]}],"id":[{"id":"10.13039\/501100000921","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","award":["101072828"],"award-info":[{"award-number":["101072828"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018694","name":"HORIZON EUROPE Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","award":["860329"],"award-info":[{"award-number":["860329"]}],"id":[{"id":"10.13039\/100018694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have established the association of sex with gene and protein expression. This study investigated the association of sex with the abundance of endogenous urinary peptides, using capillary electrophoresis\u2010coupled to mass spectrometry (CE\u2010MS) datasets from 2008 healthy individuals and patients with type II diabetes, divided in one discovery and two validation cohorts. Statistical analysis using the Mann\u2013Whitney test, adjusted for multiple testing, revealed 143 sex\u2010associated peptides in the discovery cohort. Of these, 90 peptides were associated with sex in at least one of the validation cohorts and showed agreement in their regulation trends across all cohorts. The 90 sex\u2010associated peptides were fragments of 29 parental proteins. Comparison with previously published transcriptomics data demonstrated that the genes encoding 16 of these parental proteins had sex\u2010biased expression. The 143 sex\u2010associated peptides were combined into a support vector machine\u2010based classifier that could discriminate males from females in two independent sets of healthy individuals and patients with type II diabetes, with an AUC of 89% and 81%, respectively. Collectively, the urinary peptidome contains multiple sex\u2010associated differences, which may enable a better understanding of sex\u2010biased molecular mechanisms and the development of more accurate diagnostic, prognostic, or predictive classifiers for each individual sex.<\/jats:p>","DOI":"10.1002\/pmic.202300227","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T04:31:01Z","timestamp":1695702661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Multiple urinary peptides display distinct sex\u2010specific distribution"],"prefix":"10.1002","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4099-665X","authenticated-orcid":false,"given":"Ioanna K.","family":"Mina","sequence":"first","affiliation":[{"name":"Mosaiques Diagnostics GmbH  Hannover Germany"},{"name":"Institute for Molecular Cardiovascular Research University Hospital RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanouil","family":"Mavrogeorgis","sequence":"additional","affiliation":[{"name":"Mosaiques Diagnostics GmbH  Hannover Germany"},{"name":"Institute for Molecular Cardiovascular Research University Hospital RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1407-2534","authenticated-orcid":false,"given":"Justyna","family":"Siwy","sequence":"additional","affiliation":[{"name":"Mosaiques Diagnostics GmbH  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riste","family":"Stojanov","sequence":"additional","affiliation":[{"name":"Faculty of Computer Science and Engineering Ss. Cyril and Methodius University in Skopje  Skopje North Macedonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Mischak","sequence":"additional","affiliation":[{"name":"Mosaiques Diagnostics GmbH  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8917-2412","authenticated-orcid":false,"given":"Agnieszka","family":"Latosinska","sequence":"additional","affiliation":[{"name":"Mosaiques Diagnostics GmbH  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Jankowski","sequence":"additional","affiliation":[{"name":"Institute for Molecular Cardiovascular Research University Hospital RWTH Aachen  Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2019.01.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2020.03.008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2010588118"},{"key":"e_1_2_9_5_1","first-page":"369","article-title":"The impact of sex on gene expression across human tissues","author":"Oliva M.","year":"2020","journal-title":"Science"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2017.10.002"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26237260"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062837"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-2715"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.12.010"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.202000027"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2012-302047"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2007091025"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(20)30026-7"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.115.05296"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-7568(21)00226-9"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-019-2686-0"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-594"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2012.09.025"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.200900189"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201900091"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr800401m"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-150-9-200905050-00006"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ehf2.12708"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2017.04.007"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/proteomes9030032"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-016-0246-y"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014050423"},{"key":"e_1_2_9_29_1","first-page":"2079","article-title":"Noninvasive diagnosis of chronic kidney diseases using urinary proteome analysis","volume":"32","author":"Siwy J.","year":"2017","journal-title":"Nephrology Dialysis Transplantation"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/dia.2008.0040"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2014.07.014"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v042.i08"},{"key":"e_1_2_9_33_1","doi-asserted-by":"crossref","unstructured":"An D. W. Yu Y. L. Martens D. S. Latosinska A. Zhang Z. Y. Mischak H. Nawrot T. S. &Staessen J. A.(2023).Statistical approaches applicable in managing OMICS data: Urinary proteomics as exemplary case.Mass Spectrometry Reviews.","DOI":"10.1002\/mas.21849"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2653"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-00943-x"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1206-1565"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009715923555"},{"key":"e_1_2_9_39_1","first-page":"4766","article-title":"A unified approach to interpreting model predictions","volume":"2017","author":"Lundberg S. M.","year":"2017","journal-title":"Advances in Neural Information Processing Systems"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-017-0445-z"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0040009"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e3182571e46"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/inthealth\/ihac003"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-5507-6"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c05152"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1052"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.005586"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2018.1473371"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2017.181"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11897-015-0252-x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.767785"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-031620-092817"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/nep.13688"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00278.x"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14200"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2020-021"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920207780368141"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/4.5.859"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004978"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0194458"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202300227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:00:16Z","timestamp":1761058816000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.202300227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,26]]},"references-count":60,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/pmic.202300227"],"URL":"https:\/\/doi.org\/10.1002\/pmic.202300227","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,26]]},"assertion":[{"value":"2023-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300227"}}