{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T20:43:36Z","timestamp":1789245816506,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["427588170"],"award-info":[{"award-number":["427588170"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human testicular peritubular cells (HTPCs) are smooth muscle cells, which in the testis form a small compartment surrounding the seminiferous tubules. Contractions of HTPCs are responsible for sperm transport, HTPCs contribute to spermatogenesis, have immunological roles and are a site of glucocorticoid receptor expression. Importantly, HTPCs maintain their characteristics in vitro, and thus can serve as an experimental window into the male gonad. Previously we reported consequences of 3\u2010day treatment with Dexamethasone (Dex), a synthetic glucocorticoid and multi\u2010purpose anti\u2010inflammatory drug. However, as glucocorticoid therapies in man often last longer, we now studied consequences of a prolonged 7\u2010day exposure to 1 \u00b5M Dex. Combining live cell imaging with quantative proteomics of samples taken from men, we confirmed our recent findings but more importantly, found numerous novel proteomic alterations induced by prolonged Dex treatment. The comparison of the 7\u2010day treatment with the 3\u2010day treatment dataset revealed that extracellular matrix\u2010 and focal adhesion\u2010related proteins become more prominent after 7 days of treatment. In contrast, extended stimulation is, for example, associated with a decrease of proteins related to cholesterol and steroid metabolism. Our dataset, which describes phenotypic and proteomic alterations, is a valuable resource for further research projects investigating effects of Dex on human testicular cells.<\/jats:p>","DOI":"10.1002\/pmic.202300616","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T23:46:01Z","timestamp":1709163961000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Prolonged exposure to dexamethasone alters the proteome and cellular phenotype of human testicular peritubular cells"],"prefix":"10.1002","volume":"24","author":[{"given":"Youli K.","family":"Stepanov","sequence":"first","affiliation":[{"name":"Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA) Ludwig Maximilian University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carola","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, AG Mayerhofer Ludwig Maximilian University of Munich  Planegg\u2010Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan B.","family":"St\u00f6ckl","sequence":"additional","affiliation":[{"name":"Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA) Ludwig Maximilian University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank\u2010Michael","family":"K\u00f6hn","sequence":"additional","affiliation":[{"name":"Andrologicum  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Pickl","sequence":"additional","affiliation":[{"name":"Urologie und Andrologie  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Trottmann","sequence":"additional","affiliation":[{"name":"Urologie und Andrologie  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4709-3211","authenticated-orcid":false,"given":"Thomas","family":"Fr\u00f6hlich","sequence":"additional","affiliation":[{"name":"Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA) Ludwig Maximilian University of Munich  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Mayerhofer","sequence":"additional","affiliation":[{"name":"Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, AG Mayerhofer Ludwig Maximilian University of Munich  Planegg\u2010Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Welter","sequence":"additional","affiliation":[{"name":"Biomedical Center Munich (BMC), Cell Biology, Anatomy III, Faculty of Medicine, AG Mayerhofer Ludwig Maximilian University of Munich  Planegg\u2010Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11223685"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1336.004"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1406"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-16-0588"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.59.1"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod40.4.811"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2011.01225.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400769z"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37128"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0497"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12509"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12418"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9040961"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934508"},{"issue":"2","key":"e_1_2_5_16_1","first-page":"109","article-title":"Glucocorticoids, stress, and fertility","volume":"35","author":"Whirledge S.","year":"2010","journal-title":"Minerva Endocrinologica"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.09.056"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dead187"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11193164"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.136"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-85742-0"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aao0475"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-2169"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.11.4778"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-416006-4.00011-9"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.05.031"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-20-0160"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2023.07.006"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2019.03.011"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14924"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/1008-682X.132471"},{"key":"e_1_2_5_32_1","doi-asserted-by":"crossref","unstructured":"Tanaka K. &Kusunoki M.(2011).PTMA (prothymosin alpha). Atlas of genetics and cytogenetics in oncology and haematology.https:\/\/api.semanticscholar.org\/CorpusID:84599987","DOI":"10.4267\/2042\/44643"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12091210"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/107999002320271413"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027040"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09-139998"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2007.00758.x"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24098334"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioab187"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1633516100"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg135"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2021.112799"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells2020202"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1106"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.202300616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T09:54:48Z","timestamp":1761040488000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.202300616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,28]]},"references-count":43,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/pmic.202300616"],"URL":"https:\/\/doi.org\/10.1002\/pmic.202300616","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,28]]},"assertion":[{"value":"2023-11-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2300616"}}