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Non\u2010tuberculous mycobacteria (NTM), such as <jats:italic>Mycobacterium abscessus<\/jats:italic>, are a group of environmental bacteria that can cause severe lung infections in populations with pre\u2010existing lung conditions, such as cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). There is limited knowledge of the engagement of EVs in the host\u2013pathogen interactions in the context of NTM infections. In this study, we found that <jats:italic>M<\/jats:italic><jats:italic>. abscessus<\/jats:italic> infection increased the release of a subpopulation of exosomes (CD9, CD63, and\/or CD81 positive) by mouse macrophages in cell culture. Proteomic analysis of these vesicles demonstrated that <jats:italic>M. abscessus<\/jats:italic> infection affects the enrichment of host proteins in exosomes released by macrophages. When compared to exosomes from uninfected macrophages, exosomes released by <jats:italic>M. abscessus<\/jats:italic>\u2010infected macrophages significantly improved <jats:italic>M. abscessus<\/jats:italic> growth and downregulated the intracellular level of glutamine in recipient macrophages in cell culture. Increasing glutamine concentration in the medium rescued intracellular glutamine levels and <jats:italic>M. abscessus<\/jats:italic> killing in recipient macrophages that were treated with exosomes from <jats:italic>M. abscessus<\/jats:italic>\u2010infected macrophages. Taken together, our results indicate that exosomes may serve as extracellular glutamine eliminators that interfere with glutamine\u2010dependent <jats:italic>M. abscessus<\/jats:italic> killing in recipient macrophages.<\/jats:p>","DOI":"10.1002\/pmic.202400181","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T04:19:03Z","timestamp":1726460343000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterization of Exosomes Released from <i>Mycobacterium abscessus<\/i>\u2010Infected Macrophages"],"prefix":"10.1002","volume":"25","author":[{"given":"Charlie A.","family":"Vermeire","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Oklahoma State University  Stillwater Oklahoma USA"},{"name":"Oklahoma Center for Respiratory and Infectious Diseases Oklahoma State University  Stillwater Oklahoma 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Respiratory and Infectious Diseases Oklahoma State University  Stillwater Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen K.","family":"Kotey","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Oklahoma State University  Stillwater Oklahoma USA"},{"name":"Oklahoma Center for Respiratory and Infectious Diseases Oklahoma State University  Stillwater Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Hartson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Oklahoma State University  Stillwater Oklahoma USA"},{"name":"Center for Genomics and Proteomics Oklahoma State University  Stillwater Oklahoma USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Liang","sequence":"additional","affiliation":[{"name":"Oklahoma Center for Respiratory and Infectious Diseases Oklahoma State 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