{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:15:04Z","timestamp":1782836104237,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Post\u2010acute sequelae of severe acute respiratory syndrome coronavirus 2\u2010infection (PASC) is challenging to diagnose and treat, and its molecular pathophysiology remains unclear. Urinary peptidomics can provide valuable information on urine peptides that may enable improved and specified PASC diagnosis. Using standardized capillary electrophoresis\u2010MS, we examined the urinary peptidomes of 50 patients with PASC 10 months after COVID\u201019 and 50 controls, including healthy individuals (\n                      <jats:italic>n<\/jats:italic>\n                      = 42) and patients with non\u2010COVID\u201019\u2010associated myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) (\n                      <jats:italic>n<\/jats:italic>\n                      = 8). Based on peptide abundance differences between cases and controls, we developed a diagnostic model using a support vector machine. The abundance of 195 urine peptides among PASC patients significantly differed from that in controls, with a predominant abundance of collagen alpha chains. This molecular signature (PASC195) effectively distinguished PASC cases from controls in the training set (AUC of 0.949 [95% CI 0.900\u20130.998;\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.0001]) and independent validation set (AUC of 0.962 [95% CI 0.897\u20131.00];\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.0001]). In silico assessment suggested exercise, GLP\u20101RAs and mineralocorticoid receptor antagonists (MRAs) as potentially efficacious interventions. We present a novel and non\u2010invasive diagnostic model for PASC. Reflecting its molecular pathophysiology, PASC195 has the potential to advance diagnostics and inform therapeutic interventions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Statement of Significance of the Study<\/jats:title>\n                    <jats:p>\n                      Despite the recent emergence of omics\u2010derived candidates for post\u2010acute sequelae of SARS\u2010CoV\u20102 infection (PASC), the pending validation of proposed markers and lack of consensus result in the continuous reliance on symptom\u2010based criteria, being subject to diagnostic uncertainties and potential recall bias. Building upon prior findings of renal involvement in acute COVID\u201019 pathophysiology and PASC\u2010associated alterations, we hypothesized that the use of urinary peptides for PASC\u2010specific biomarker discovery, unlike conventional specimens that have been utilized thus far, may offer complementary information on putative disease mechanisms. In the present study, 195 significantly expressed peptides were used to form a classifier termed PASC195, which effectively discriminated PASC from non\u2010PASC (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.0001), including healthy individuals and non\u2010COVID\u201019\u2010associated myalgic encephalomyelitis\/chronic fatigue syndrome, in both the derivation (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a060) and an independent validation set (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a040). The peptidome profile associated with PASC was consistent with a shift in collagen turnover, with most PASC195 peptides derived from alpha chains. Ongoing inflammatory responses, hemostatic imbalances, and endothelial damage were indicated by cross\u2010sectional variations in endogenous peptide excretion.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pmic.70074","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T07:43:31Z","timestamp":1763797411000},"page":"32-43","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Urinary Peptidomic Profiling In Post\u2010Acute Sequelae of SARS\u2010CoV\u20102 Infection: A Case\u2010Control Study"],"prefix":"10.1002","volume":"26","author":[{"given":"Dilara","family":"G\u00fclmez","sequence":"first","affiliation":[{"name":"Department of Epidemiology Center for Public Health Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Siwy","sequence":"additional","affiliation":[{"name":"Mosaiques Diagnostics GmbH Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Kurz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II (Infectious Diseases, Immunology, Rheumatology, Pneumology) Medical University of Innsbruck Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Wendt","sequence":"additional","affiliation":[{"name":"Division of Nephrology St. Georg Hospital Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslaw","family":"Banasik","sequence":"additional","affiliation":[{"name":"Department of Nephrology Transplantation Medicine and Internal Diseases Wroc\u0142aw Medical University Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Molecular and Clinical Medicine Institute of Medicine the Sahlgrenska Academy at University of Gothenburg Gothenburg Sweden"},{"name":"Region V\u00e4stra G\u00f6taland Skaraborg Hospital Department of Nephrology Sk\u00f6vde Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Dudoignon","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Critical Care H\u00f4pital Saint Louis \u2010 Lariboisi\u00e8re AP\u2010HP Universite Paris Cit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Depret","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Critical Care H\u00f4pital Saint Louis \u2010 Lariboisi\u00e8re AP\u2010HP Universite Paris Cit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Salgueira","sequence":"additional","affiliation":[{"name":"Virgen Macarena Hospital University of Seville Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Nowacki","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Des Patients \u2013 Sorbonne Universit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelie","family":"Kurnikowski","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Center for Public Health Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Mussnig","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Center for Public Health Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Krenn","sequence":"additional","affiliation":[{"name":"IUVABIT e.U. Analytics &amp; Data Science Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Gonos","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II (Infectious Diseases, Immunology, Rheumatology, Pneumology) Medical University of Innsbruck Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"L\u00f6ffler\u2010Ragg","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II (Infectious Diseases, Immunology, Rheumatology, Pneumology) Medical University of Innsbruck Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter","family":"Weiss","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II (Infectious Diseases, Immunology, Rheumatology, Pneumology) Medical University of Innsbruck Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Mischak","sequence":"additional","affiliation":[{"name":"Mosaiques Diagnostics GmbH Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Hecking","sequence":"additional","affiliation":[{"name":"Department of Nephrology Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Schernhammer","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Center for Public Health Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Beige","sequence":"additional","affiliation":[{"name":"Division of Nephrology St. Georg Hospital Leipzig Germany"},{"name":"Kuratorium For Dialysis and Transplantation (KfH) Leipzig Leipzig Germany"},{"name":"Department of Internal Medicine II Martin\u2010Luther\u2010University Halle\u2010Wittenberg Halle Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"UriCoV Working Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.12603"},{"key":"e_1_2_13_3_1","unstructured":"COVID\u201019 Deaths | WHO COVID\u201019 Dashboard. 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