{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T10:13:06Z","timestamp":1787739186910,"version":"build-2784847793"},"reference-count":23,"publisher":"Wiley","issue":"2-3","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82203711"],"award-info":[{"award-number":["82203711"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M701631"],"award-info":[{"award-number":["2021M701631"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Prostate cancer (PCa) is a leading male malignancy worldwide, with metabolic reprogramming being a critical hallmark of its progression. Extracellular vesicles (EVs) derived from tissues directly reflect the tumor microenvironment, offering unique insights into cancer pathophysiology that are unattainable through cell line or biofluid\u2010derived EVs. However, the functional roles of tissue\u2010derived EVs in PCa metabolism remain poorly understood. Leveraging our expertise in murine PCa model establishment and EV isolation from prostate tissue, this study aimed to characterize functional differences between PCa and normal prostate tissue via proteomic analysis of tissue\u2010derived sEVs. We orthotopically implanted luciferase\u2010labeled PCa cells into nude mice to establish an in situ PCa model, confirmed tumor formation via in vivo imaging, and harvested tissues after 4 weeks. sEVs were isolated using ultracentrifugation combined with an iodixanol density cushion and characterized by transmission electron microscopy, nanoparticle tracking analysis, and protein marker profiling. Proteomic analysis identified 28 upregulated and 24 downregulated proteins in PCa\u2010derived sEVs compared to normal controls. Subcellular localization revealed enrichment in the cytoplasm, while pathway analysis highlighted significant involvement in metabolic processes, particularly glycolysis, amino acid biogenesis, carbon metabolism, and pyruvate metabolism. Our study establishes a robust method for isolating prostate tissue sEVs and provides the first evidence that PCa tissue\u2010derived sEVs exhibit profound metabolic pathway alterations. These findings enhance our understanding of PCa progression mechanisms and may facilitate the development of novel diagnostic biomarkers and therapeutic strategies targeting metabolic dysregulation in PCa.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Summary<\/jats:title>\n                    <jats:p>In this study, we created a method to isolate prostate tissue small EVs, based on our knowledge of the murine prostate cancer model building. Our data suggested that prostate tissue small EVs proteins significantly changed in many metabolism pathways, such as Glycolysis, Biogenesis of amino acids, Carbon metabolism and Pyruvate metabolism. In this study, we are the first to report prostate tissue\u2010derived EVs proteins enriched in alterations of cancer metabolism. These differential proteins in PCa tissue EVs reflect metabolic changes in PCa and may provide insights into the development of early diagnostic biomarkers or novel therapeutic strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pmic.70081","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T09:02:43Z","timestamp":1763370163000},"page":"121-128","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Proteomics of Prostate Cancer Tissue Small Extracellular Vesicles Reveal Alteration of Metabolism"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3558-3800","authenticated-orcid":false,"given":"Lijuan","family":"Yu","sequence":"first","affiliation":[{"name":"Sahlgrenska Center for Cancer Research and Wallenberg Centre for Molecular and Translational Medicine Department of Surgery Institute of Clinical Sciences University of Gothenburg  Gothenburg Sweden"},{"name":"Department of Laboratory Medicine Guangdong Provincial Key Laboratory of Precision Medical Diagnostics Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors Guangdong Provincial Key Laboratory of Single\u2010cell and Extracellular Vesicles Nanfang Hospital Southern Medical University  Guangzhou P. R. China"},{"name":"Institute of Laboratory Medicine Center of Chinese People's Liberation Army (PLA) Xijing Hospital Fourth Military Medical University (Air Force Medical University)  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The Second Hospital Affiliated to Xi'an Jiaotong University Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Olofsson Bagge","sequence":"additional","affiliation":[{"name":"Sahlgrenska Center for Cancer Research and Wallenberg Centre for Molecular and Translational Medicine Department of Surgery Institute of Clinical Sciences University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Guangdong Provincial Key Laboratory of Precision Medical Diagnostics Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors Guangdong Provincial Key Laboratory of Single\u2010cell and Extracellular Vesicles Nanfang Hospital Southern Medical University  Guangzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoke","family":"Hao","sequence":"additional","affiliation":[{"name":"College of Medicine Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2022.100273"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12175"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2020.1722433"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010020\u201000466\u20101"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.07.009"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.01.007"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12056"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717363115"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20190158"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.49186"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4185"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/INMD.20230016"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2753"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3169"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15756"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010019\u20100404\u20104"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2020.1736935"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/07357907.2015.1081921"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2025.100944"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010020\u201003729\u20103"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201802675R"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20190158"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.70081","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pmic.70081","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.70081","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:44:50Z","timestamp":1783014290000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.70081"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,17]]},"references-count":23,"journal-issue":{"issue":"2-3","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/pmic.70081"],"URL":"https:\/\/doi.org\/10.1002\/pmic.70081","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,17]]},"assertion":[{"value":"2025-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}