{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:07Z","timestamp":1786978387104,"version":"build-2736575974"},"reference-count":74,"publisher":"Wiley","issue":"2-3","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Seminal extracellular vesicles (SEVs) carry a diverse array of bioactive molecules, including proteins, lipids, and nucleic acids, which influence sperm function and have potential to modulate the female reproductive tract immune response after intromission. However, the full spectrum of SEV cargo involved in these processes remains incompletely defined. Here, we employed label\u2010free quantitative high\u2010resolution mass spectrometry to characterize the human SEV proteome, identifying 5079 associated proteins. These proteins were shown to likely originate from multiple regions of the male reproductive tract, notably the seminal vesicles and prostate, providing evidence for heterogeneous tissue origins of SEVs. Bioinformatic analysis revealed enrichment in sperm\u2010 and immune\u2010related functions, as well as functions linked to protein translation. Notably, we identified several proteins with established roles in sperm physiology and immune signaling that had not previously been linked with SEV function. These included; Adenylate kinase isoenzyme (AK)2\/9, and Calcium\u2010binding tyrosine\u2010phosphorylation regulated protein (CABYR), implicated in sperm motility, and immune regulators such as the Toll\u2010like receptor 4 ligand, High mobility group protein B1 (HMGB1), and the Nuclear factor kappa\u2010light\u2010chain\u2010enhancer of activated B cells (NF\u03baB) inhibitor epsilon (NF\u03baBIE). Altogether, these findings expand the known SEV proteome and highlight proteins that may influence both male and female reproductive capacity.<\/jats:p>","DOI":"10.1002\/pmic.70088","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T01:24:17Z","timestamp":1765589057000},"page":"178-188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Advancing Understanding of the Protein Composition of Human Seminal Extracellular Vesicles"],"prefix":"10.1002","volume":"26","author":[{"given":"Cottrell T.","family":"Tamessar","sequence":"first","affiliation":[{"name":"Centre for Reproductive Science, School of Environmental and Life Sciences, College of Engineering, Science and Environment, University of Newcastle University Drive Callaghan NSW Australia"},{"name":"Infertility and Reproduction Research Program Hunter Medical Research Institute  New Lambton Heights NSW 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Newcastle University Drive Callaghan NSW Australia"},{"name":"Infertility and Reproduction Research Program Hunter Medical Research Institute  New Lambton Heights NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A.","family":"Robertson","sequence":"additional","affiliation":[{"name":"The Robinson Research Institute and School of Biomedicine University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth G.","family":"Bromfield","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Science, School of Environmental and Life Sciences, College of Engineering, Science and Environment, University of Newcastle University Drive Callaghan NSW Australia"},{"name":"Infertility and Reproduction Research Program Hunter Medical Research Institute  New Lambton Heights NSW Australia"},{"name":"Bio21 Institute School of BioSciences University of Melbourne  VIC 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