{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T18:52:11Z","timestamp":1783018331174,"version":"3.54.6"},"reference-count":30,"publisher":"Wiley","issue":"2-3","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009438","name":"National Center of Neurology and Psychiatry","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009438","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We systematically reviewed published studies to assess reproducibility in miRNA expression profiles from extracellular vesicles (EVs) isolated from mouse serum. Our search specifically targeted mouse studies employing precipitation methods for EV isolation from blood and small RNA sequencing of EV\u2010miRNAs in control groups. Out of 53 identified studies, approximately half lacked publicly available raw data, leaving four eligible studies containing sequencing data from a total of 11 mice. miRNA expression counts were standardized using\n                    <jats:italic>z<\/jats:italic>\n                    \u2010scores for comparability. Within individual studies, miRNA profiles showed reasonable consistency; however, significant variability was observed across different studies. Principal component analysis (PCA), t\u2010distributed stochastic neighbor embedding (t\u2010SNE), and Spearman correlation consistently demonstrated study\u2010specific clustering rather than biological similarity. Methodological discrepancies in EV isolation, RNA extraction protocols, and unreported confounders such as platelet contamination or blood\u2010handling procedures likely contributed to this variability. Our findings emphasize substantial reproducibility challenges in EV\u2010miRNA research across murine studies, highlighting an urgent need for standardized methodologies and transparent reporting to improve reliability of miRNA biomarker discovery and facilitate meta\u2010analytic integration in preclinical research.\n                  <\/jats:p>","DOI":"10.1002\/pmic.70102","type":"journal-article","created":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T12:06:44Z","timestamp":1767096404000},"page":"154-162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessing Discrepancies in miRNA Profiles of Mouse Blood\u2010Derived Extracellular Vesicles: A Systematic Review of Sequencing Data"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9726-1009","authenticated-orcid":false,"given":"Md Sorwer Alam","family":"Parvez","sequence":"first","affiliation":[{"name":"Department of 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Hase","sequence":"additional","affiliation":[{"name":"Center for Education in Healthcare Innovation Institute of Science Tokyo  Tokyo Japan"},{"name":"The Systems Biology Institute  Shinagawa\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoru","family":"Mogushi","sequence":"additional","affiliation":[{"name":"Center for Education in Healthcare Innovation Institute of Science Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5365-4900","authenticated-orcid":false,"given":"Eisuke","family":"Dohi","sequence":"additional","affiliation":[{"name":"Center for Education in Healthcare Innovation Institute of Science Tokyo  Tokyo Japan"},{"name":"National Center of Neurology and Psychiatry National Institute of Neuroscience  Kodaira Tokyo 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