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In this study, we investigated the molecular mechanisms underlying the anticancer effects of the nucleolin (NCL)\u2010targeting DNA aptamer AS1411 using label\u2010free quantitative proteomic profiling. AS1411 treatment significantly reduced TNBC cell viability and migration. To uncover the underlying mechanisms, we performed global proteomic analysis of AS1411\u2010treated TNBC cells. Bioinformatic analysis of differentially expressed proteins (DEPs) revealed enrichment of tumor\u2010associated signaling pathways and protein\u2013protein interaction networks regulated by AS1411. Among the DEPs, ATPase H\n                    <jats:sup>+<\/jats:sup>\n                    \u2010transporting accessory protein 1 (ATP6AP1) was markedly downregulated in AS1411\u2010treated TNBC cells. Functional studies demonstrated that ATP6AP1 knockdown suppressed TNBC cell proliferation and migration, whereas its overexpression enhanced tumorigenic phenotypes. Importantly, modulation of ATP6AP1 expression showed minimal effects on normal breast epithelial cells. Collectively, these findings identify ATP6AP1 as a key downstream mediator of AS1411 and support its potential as a therapeutic target in TNBC.\n                  <\/jats:p>","DOI":"10.1002\/pmic.70122","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T17:39:54Z","timestamp":1774633194000},"page":"28-41","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic Characterization of AS1411 Reveals ATP6AP1 as a Mediator of Triple\u2010Negative Breast Cancer Progression"],"prefix":"10.1002","volume":"26","author":[{"given":"Ha\u2010Song","family":"Bae","sequence":"first","affiliation":[{"name":"Graduate School of Analytical Science and Technology (GRAST) Chungnam National University  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Jeong","family":"Seo","sequence":"additional","affiliation":[{"name":"Graduate School of Analytical Science and Technology (GRAST) Chungnam National University  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun\u2010Bi","family":"Kim","sequence":"additional","affiliation":[{"name":"Graduate School of Analytical Science and Technology (GRAST) Chungnam National University  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7397-8286","authenticated-orcid":false,"given":"Hyoung\u2010Min","family":"Park","sequence":"additional","affiliation":[{"name":"Biometrology group, Division of Biomedical Metrology Korea Research Institute of Standards and Science Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4524-3263","authenticated-orcid":false,"given":"I\u2010Hsuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology National Taiwan University  Taipei City Taiwan (R.O.C.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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