{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:18:44Z","timestamp":1790309924878,"version":"4.1.0"},"reference-count":12,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The advent of proteomics assays that provide accurate levels of thousands of proteins from relatively small sample volumes opens possibilities for biomarker research in biobanks with valuable samples. In this study, we leverage the applicability of the Olink proximity extension assay to profile serum samples of the Janus Serum Bank, which have been stored at \u201325\u00b0C for several decades. We perform association analysis for the body mass index, sex, smoking status and storage time, and compare these associations against protein associations of these traits in the UK Biobank. We identify significant and plausible associations of proteins levels for the analysed phenotypes. Furthermore, these associations showed a high degree of correlation to the analyses performed on the large\u2010scale studies by the UK Biobank. The samples obtained in the UK Biobank were collected according to modern standards, with short processing time for the blood samples, and storage at very low temperatures, whereas samples in the Janus Serum Bank were collected over larger timespans, with storage in sub\u2010optimal temperature and over prolonged periods of time as compared to todays\u2019 standards. This research shows that samples stored under sub\u2010optimal conditions and over extreme periods of time (up to 50 years) yield reliable protein associations for common traits using a proximity extension assay. Therefore, archived biosamples that were not stored in optimal conditions, including samples of the Janus Serum Bank, remain a highly valuable resource in disease and risk biomarker research using modern and emerging proteomics assay methods.<\/jats:p>","DOI":"10.1002\/pmic.70145","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:49:07Z","timestamp":1779342547000},"page":"106-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomic Profiling Using a Proximity Extension Assay Panel on Serum Samples Stored at \u221225\u00b0C Over Several Decades"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2975-7520","authenticated-orcid":false,"given":"Steffan Daniel","family":"Bos","sequence":"first","affiliation":[{"name":"Department of Research Cancer Registry of Norway Norwegian Institute of Public Health  Oslo Norway"},{"name":"Department of Microbiology Haukeland University Hospital  Bergen Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3806-2296","authenticated-orcid":false,"given":"Sigr\u00fan Helga","family":"Lund","sequence":"additional","affiliation":[{"name":"deCODE genetics\/Amgen Inc.  Reykjavik Iceland"},{"name":"Faculty of Physical Sciences University of Iceland  Reykjavik Iceland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1426-8505","authenticated-orcid":false,"given":"Ren\u00e9e Turzanski","family":"Fortner","sequence":"additional","affiliation":[{"name":"Department of Research Cancer Registry of Norway Norwegian Institute of Public Health  Oslo Norway"},{"name":"Division of Cancer Epidemiology German Cancer Research Center  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9103-1077","authenticated-orcid":false,"given":"Eivind","family":"Hovig","sequence":"additional","affiliation":[{"name":"Department of Tumor Biology Institute for Cancer Research Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9446-4855","authenticated-orcid":false,"given":"Hilde","family":"Langseth","sequence":"additional","affiliation":[{"name":"Department of Research Cancer Registry of Norway Norwegian Institute of Public Health  Oslo Norway"},{"name":"Department of Epidemiology and Biostatistics School of Public Health Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2021.100168"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015004"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006563\u2010x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006592\u20106"},{"issue":"2","key":"e_1_2_11_6_1","first-page":"403","article-title":"Cohort Profile: The Janus Serum Bank Cohort in Norway","volume":"46","author":"Langseth H.","year":"2017","journal-title":"International Journal of Epidemiology"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/19475535251371418"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm\u20102016\u20100892"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0277\u20105379(90)90049\u2010Y"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.968815"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.1971.tb00013.x"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2025.101000"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201048017\u20106"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.70145","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pmic.70145","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.70145","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T06:24:41Z","timestamp":1787811881000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.70145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":12,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/pmic.70145"],"URL":"https:\/\/doi.org\/10.1002\/pmic.70145","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]},"assertion":[{"value":"2025-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}