{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T10:30:40Z","timestamp":1786530640445,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Serum small extracellular vesicles (sEVs) carry glycoproteins that reflect the pathophysiological states of their cells of origin, yet their glycomic profiles in neuropsychiatric disorders remain largely unexplored. Here, we isolated sEVs from healthy controls and patients with depressive disorder (DD), schizophrenia (SCZ), or brain glioma (BG), and characterized their glycopatterns using a high\u2010throughput lectin microarray. Concentrations and CD81 expression of sEVs were significantly elevated across all disease groups relative to healthy controls. Lectin microarray analysis revealed both shared and disease\u2010specific glycan alterations: PSA\u2010recognized glycans (high\u2010mannose and core\u2010fucosylated structures) were consistently downregulated in all three conditions, whereas STA\u2010binding glycans (GlcNAc oligomers and core GlcNAc of N\u2010glycans) were upregulated in SCZ and BG but slightly decreased in DD. Disease\u2010specific lectin signatures\u2014including UEA\u2010I, PHA\u2010L, and DBA in DD, and ACA in SCZ\u2014enabled clear discrimination among the four groups. The STA\/PSA fluorescence ratio, validated in individual samples via sEVs microarray, effectively distinguished SCZ and BG from DD and controls. These findings establish that serum sEVs harbor disease\u2010specific glycopatterns capable of differentiating depression, schizophrenia, and glioblastoma, offering a minimally invasive diagnostic approach and providing insights into glycan\u2010related mechanisms underlying these disorders.<\/jats:p>","DOI":"10.1002\/pmic.70170","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T09:40:56Z","timestamp":1786527656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Disease\u2010Specific Glycopattern Signatures of Serum Small Extracellular Vesicles in Depression, Schizophrenia, and Glioblastoma"],"prefix":"10.1002","author":[{"given":"Jinye","family":"Gong","sequence":"first","affiliation":[{"name":"Institute of Genetics and Development Biology Translational Medicine Institute &amp; School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonglin","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Development Biology Translational Medicine Institute &amp; School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangbo","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Neurology Xianyang Hospital of Yan'an University  Xianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Development Biology Translational Medicine Institute &amp; School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Anas","family":"Ramzan","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Development Biology Translational Medicine Institute &amp; School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Development Biology Translational Medicine Institute &amp; School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Liu","sequence":"additional","affiliation":[{"name":"Environment and Genes Related to Diseases Key Laboratory of Education Ministry Xi'an Jiaotong University Health Science Center  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannan","family":"Qin","sequence":"additional","affiliation":[{"name":"Institute of Genetics and Development Biology Translational Medicine Institute &amp; School of Basic Medical Sciences Xi'an Jiaotong University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"issue":"20","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"4485","DOI":"10.1080\/10408398.2021.2002806","article-title":"Prevalence of Food Insecurity in People With Major Depression, Bipolar Disorder, and Schizophrenia and Related Psychoses: A Systematic Review and Meta\u2010Analysis","volume":"63","author":"Teasdale S. 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