{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T06:06:34Z","timestamp":1789538794241,"version":"build-2803163510"},"reference-count":10,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Tape\u2010stripping of the stratum corneum is a promising non\u2010invasive approach for proteomic analysis of skin lesions, but the effects of sampling design and disc pressure on proteome readout remain poorly defined. Here, we assessed how inclusion of surrounding normal\u2010looking skin and variation in adhesive disc pressure influence protein detection from small melanocytic lesions. D\u2010squame tape strips were collected from six participants, including three moles and one matched normal skin site per participant. For lesion 1 (L1), discs were applied using light finger pressure. For lesion 2 (L2), the mole area was marked on the disc and separated from the surrounding skin, which was analysed separately (L2S). For lesion 3 (L3), increased pressure was applied using the upper barrel tip of a marker before excising the lesion\u2010matched area. Samples were analysed by DIA\u2010MS with DIA\u2010NN processing. In total, 2882 protein groups were identified, including 1719 shared across all groups. L3 yielded the highest number of unique proteins (77) and significantly altered proteins versus normal skin (142), compared with L2 (54 unique; 52 altered), L1 (30 unique; 4 altered), L2S (16 unique; 3 altered), and normal skin (7 unique). These findings show that precise lesion\u2010matched disc trimming and increased application pressure substantially improve tape\u2010strip proteomic depth and lesion\u2010specific signal detection. Multivariate analysis and supervised classification further separated lesional samples\u2014most clearly the pressure\u2010assisted L3\u2014from other collection methods, supporting these observations, although the small cohort warrants cautious interpretation.<\/jats:p>","DOI":"10.1002\/pmic.70177","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:55:27Z","timestamp":1789538127000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tape\u2010Stripped Proteomics: A Comparative Analysis of Different Sample Collection Approaches"],"prefix":"10.1002","author":[{"given":"Ali","family":"Azimi","sequence":"first","affiliation":[{"name":"Department of Dermatology Westmead Hospital University of Sydney  Sydney New South Wales Australia"},{"name":"Centre For Cancer Research The Westmead Institute For Medical Research Sydney New South Wales Australia"},{"name":"Department of Dermatolgoy, Westmead Hospital Westmead Clinical School Faculty of Medicine and Health The University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Faul","sequence":"additional","affiliation":[{"name":"Department of Dermatology Westmead Hospital University of Sydney  Sydney New South Wales Australia"},{"name":"Centre For Cancer Research The Westmead Institute For Medical Research Sydney New South Wales Australia"},{"name":"Department of Dermatolgoy, Westmead Hospital Westmead Clinical School Faculty of Medicine and Health The University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petrina","family":"Chand","sequence":"additional","affiliation":[{"name":"Department of Dermatology Westmead Hospital University of Sydney  Sydney New South Wales Australia"},{"name":"Centre For Cancer Research The Westmead Institute For Medical Research Sydney New South Wales Australia"},{"name":"Department of Dermatolgoy, Westmead Hospital Westmead Clinical School Faculty of Medicine and Health The University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Fernandez\u2010Penas","sequence":"additional","affiliation":[{"name":"Department of Dermatology Westmead Hospital University of Sydney  Sydney New South Wales Australia"},{"name":"Centre For Cancer Research The Westmead Institute For Medical Research Sydney New South Wales Australia"},{"name":"Department of Dermatolgoy, Westmead Hospital Westmead Clinical School Faculty of Medicine and Health The University of Sydney  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14921"},{"issue":"11","key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"6140","DOI":"10.3390\/ijms23116140","article-title":"Profiling the Atopic Dermatitis Epidermal Transcriptome by Tape Stripping and BRB\u2010seq","volume":"23","author":"Hu T.","year":"2022","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.202200039"},{"issue":"8","key":"e_1_2_5_5_1","doi-asserted-by":"crossref","DOI":"10.1111\/exd.15154","article-title":"Simple Tape\u2010Stripping Method for Highly Reliable and Quantitative Analysis of Skin Microbiome","volume":"33","author":"Soga N.","year":"2024","journal-title":"Experimental Dermatology"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2022.12.024"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102133.2011.10239.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2024.105329"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100638\u2010x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201017\u20100802"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1080"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.70177","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pmic.70177","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.70177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:55:28Z","timestamp":1789538128000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.70177"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":10,"alternative-id":["10.1002\/pmic.70177"],"URL":"https:\/\/doi.org\/10.1002\/pmic.70177","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"2026-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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