{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:47:22Z","timestamp":1792061242855,"version":"4.4.0"},"reference-count":71,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Proteomics is a powerful tool for profiling the protein landscape underlying cellular and physiological processes. Advances in mass spectrometry\u2010based methods, such as tandem mass tags (TMT) isobaric labeling\u2010based proteomics at both MS2 and MS3, label\u2010free quantification (LFQ) with data\u2010dependent acquisition (DDA) or data\u2010independent acquisition (DIA)\u2010based proteomics, and stable isotope labeling with amino acid (SILAC) spike\u2010in\u2010assisted proteomics, enable quantitative proteome comparisons across biological conditions. These five quantitative proteomic methods offer distinct advantages in coverage, throughput, and quantitative reproducibility. Here, we treated them as orthogonal approaches, retaining protein candidates with significant, consistent\u2010direction changes supported across multiple independently instrumented methods to identify high\u2010confidence protein\u2010level changes associated with longevity. Insulin\/insulin\u2010like growth factor signaling (IIS) is a highly conserved longevity regulatory pathway from\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    to humans. By quantitatively profiling IIS mutants in\n                    <jats:italic>C. elegans<\/jats:italic>\n                    and performing genetic screening, we obtained a systems\u2010view of proteomic changes linked to longevity and identified new longevity regulators. This systematic cross\u2010method comparison also serves as a valuable resource for guiding the selection of quantitative proteomics approaches.\n                  <\/jats:p>","DOI":"10.1002\/pmic.70195","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:11:16Z","timestamp":1792059076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Proteomic Quantification Reveals Novel Longevity Regulators"],"prefix":"10.1002","author":[{"given":"Lang","family":"Ding","sequence":"first","affiliation":[{"name":"Janelia Research Campus Howard Hughes Medical Institute Ashburn Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanfeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Graduate Program in Chemical, Physical &amp; Structural Biology, Graduate School of Biomedical Science Baylor College of Medicine Houston Texas 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Rose","family":"Pitts","sequence":"additional","affiliation":[{"name":"Janelia Research Campus Howard Hughes Medical Institute Ashburn Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayse Sena","family":"Mutlu","sequence":"additional","affiliation":[{"name":"Huffington Center on Aging Baylor College of Medicine  Houston Texas USA"},{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhou","sequence":"additional","affiliation":[{"name":"Huffington Center on Aging Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Ma","sequence":"additional","affiliation":[{"name":"Huffington Center on Aging Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Min","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine  Houston Texas USA"},{"name":"Lester and Sue Smith Breast Center Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Jin","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology Baylor College of Medicine  Houston Texas USA"},{"name":"Center for NextGen Therapeutics Baylor College of Medicine  Houston Texas USA"},{"name":"Department of Molecular and Cellular Biology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng C.","family":"Wang","sequence":"additional","affiliation":[{"name":"Janelia Research Campus Howard Hughes Medical Institute Ashburn Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470015902.a0006188.pub2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201006592\u20106"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62585"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb.2011.37"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1675"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1679"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2016.06.011"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0262560"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.08.025"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.03.032"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M113.031591"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010018\u20100006\u20109"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1714"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M200025\u2010MCP200"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010018\u201003309\u20106"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201800650"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.044305"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100638\u2010x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201030094\u20100"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20178126"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq054"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010021\u201000968\u20107"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3255"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010018\u20100003\u20105"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c01155"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08980"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/366461a0"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5328.942"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139952"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1322"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015283\u2010z"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4256"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00544"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201500488"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3901"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.TIR119.001646"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx364"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201047899\u2010w"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42004\u2010025\u201001753\u20102"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.119.302919"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1046\u20102023(03)00050\u20101"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2505604"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000579"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA119.001714"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00153"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr068288j"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmeasuresciau.4c00007"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr3003533"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010012\u20106203\u20104"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010007\u20101486\u20106"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1000"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14021"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406207200"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2021.03.034"},{"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