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The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP\u20102 and IGF\u20101\u2010loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP\u20102 and IGF\u20101 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA\u2010PEG\u2010COOH MPs is in the range of 64.78% to 76.11%. E\u2010beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E\u2010beam sterilization decreases the bioactivity of growth factors in MPs by \u224822%. BMP\u20102 release behavior from gelatin MPs\/PEG hydrogel shows a faster release (52.7%) than that of IGF\u20101 from the PLGA\u2010PEG\u2010COOH MPs\/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA\u2010PEG\u2010COOH MPs\u2010based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors\u2010loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.<\/jats:p>","DOI":"10.1002\/ppsc.202000180","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T20:06:40Z","timestamp":1598558800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Development of PLGA\u2010PEG\u2010COOH and Gelatin\u2010Based Microparticles Dual Delivery System and E\u2010Beam Sterilization Effects for Controlled Release of BMP\u20102 and IGF\u20101"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4692-624X","authenticated-orcid":false,"given":"Yan","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University  California CA 94305 USA"},{"name":"School of Pharmacy Chongqing Medical University  Chongqing 400016 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyedsina","family":"Moeinzadeh","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University  California CA 94305 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungwoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University  California CA 94305 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youngbum","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University  California CA 94305 USA"},{"name":"Department of Prosthodontics Yonsei University College of Dentistry  Seoul 120\u2010752 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine","family":"Lui","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University  California CA 94305 USA"},{"name":"Department of Mechanical Engineering Stanford University  California CA 94305 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Biomedical Informatics The University of Texas Health Science Center at Houston  Houston TX 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiling","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Biomedical Informatics The University of Texas Health Science Center at Houston  Houston TX 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaobo","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Biomedical Informatics The University of Texas Health Science Center at Houston  Houston TX 77030 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunzhi Peter","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Stanford University  California CA 94305 USA"},{"name":"Department of Materials Science and Engineering Stanford University  California CA 94305 USA"},{"name":"Department of Bioengineering Stanford University  California CA 94305 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,18]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.04.053"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12257-017-0436-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.20144"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2009.10.020"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.05.034"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.2004.01303.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-013-0538-6"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(02)00046-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-012-1084-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2003.11.014"},{"key":"e_1_2_7_11_1","first-page":"567322","volume":"7","author":"Zhang J.","year":"2017","journal-title":"RSC Adv."},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.07.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.01.009"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2017.10.008"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/7\/5\/054102"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/B978-1-78242-087-3.00010-9","volume-title":"Characterization and Design of Tissue Scaffolds","author":"Baume A. 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