{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T20:42:00Z","timestamp":1774125720522,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,12,2]],"date-time":"2002-12-02T00:00:00Z","timestamp":1038787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inhaled nitric oxide (NO) is a selective vasodilator in pulmonary hypertension. However, the safety of inhaled NO (iNO) has not been established. Using an immunohistochemical technique, we studied the expression of NO synthase (NOS) isoforms NOS1, NOS2, NOS3, and nitrotyrosine, the marker of toxic NO\u2010superoxide pathway, in lung specimens from autopsies.<\/jats:p><jats:p>Twelve infants dying with respiratory failure had iNO up to 60 parts per million for 0.1\u201315 days. Twelve control infants were matched in pairs on the basis of the diagnosis, number of gestational days at birth, age at death, and whether extracorporeal perfusion was required. In addition, 5 infants who died of SIDS or nonpulmonary trauma (healthy lungs) were compared to 8 age\u2010matched cases with respiratory failure. Immunostaining was graded by the intensity of the color deposit and the frequency in specific cells stained.<\/jats:p><jats:p>Inhaled NO tended to increase NOS2 expression in bronchiolar epithelium and adjacent tissue. There were no other differences in the distribution of nitrotyrosine or NOS isoforms between iNO\u2010treated infants and the control group with respiratory failure. All NOS isoforms were evident in the lungs studied. In severe respiratory failure, nitrotyrosine was mostly detectable in the bronchiolar epithelium and alveolar exudates, whereas in healthy lungs those sites did not contain nitrotyrosine.<\/jats:p><jats:p>The alveolar tissue of infants with progressive respiratory may be affected by the NO\u2010superoxide pathway. However, inhalation of NO was not associated with a detectable increase in oxidant stress. Pediatr Pulmonol. 2003; 35:8\u201316. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ppul.10222","type":"journal-article","created":{"date-parts":[[2002,12,3]],"date-time":"2002-12-03T13:53:35Z","timestamp":1038923615000},"page":"8-16","source":"Crossref","is-referenced-by-count":10,"title":["Nitrotyrosine and NO synthases in infants with respiratory failure: Influence of inhaled NO"],"prefix":"10.1002","volume":"35","author":[{"given":"Outi","family":"Aikio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katri","family":"Vuopala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marja\u2010Leena","family":"Pokela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sture","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikko","family":"Hallman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12,2]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.001135"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(99)70196-4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0146-0005(96)80046-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.149.2.7508323"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/7.5.471"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1281928"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.5.C1424"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117607"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.275.6.L1031"},{"key":"e_1_2_1_11_2","first-page":"1250","article-title":"Evidence for in vivo peroxynitrite production in human acute lung injury","volume":"151","author":"Kooy NW","year":"1995","journal-title":"Am J Respir Crit Care Med"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.155.5.9154889"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.98.11040809"},{"key":"e_1_2_1_14_2","first-page":"383","article-title":"Nitric oxide synthase isoform expression in the developing lung epithelium","volume":"276","author":"Sherman TS","year":"1999","journal-title":"Am J Physiol"},{"key":"e_1_2_1_15_2","first-page":"88","article-title":"Developmental expression of NOS isoforms in fetal rat lung: implications for transitional circulation and pulmonary angiogenesis","volume":"270","author":"Xue C","year":"1996","journal-title":"Am J Physiol"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.105.5.1013"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.160.3.9810048"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(98)70312-9"},{"key":"e_1_2_1_19_2","first-page":"509","volume-title":"Neonatology. 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