{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T06:37:53Z","timestamp":1781937473991,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,6,14]],"date-time":"2001-06-14T00:00:00Z","timestamp":992476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We recently reported that prenatal glucocorticoid therapy is less effective at promoting an improvement in lung function in male than in female sheep. This observation, and the higher incidence of respiratory distress syndrome in human males, suggests that the male fetal lung may be less responsive to glucocorticoids than is the female fetal lung. Since glucocorticoids are known to exert their effects via specific cytoplasmic glucocorticoid receptors (GR), we hypothesized that there may be sexual dimorphism in either the number or binding affinity of lung GR. To test the hypothesis, binding of dexamethasone (a synthetic glucocorticoid, 0.5\u201340 nM) by cytosolic fractions of male (n\u2009=\u200916) and female (n\u2009=\u200916) fetal sheep lung was measured at 125 days gestation (term\u2009=\u2009148 days).<\/jats:p><jats:p>Scatchard analysis of dexamethasone binding showed that the total number of GR (Bmax) did not significantly differ between male (346\u2009\u00b1\u200942 fmol\/mg protein) and female (277\u2009\u00b1\u200923 fmol\/mg protein) fetuses. The measured binding affinity (Kd) in male fetal lungs (6.85\u2009\u00b1\u20090.43 nM) was not significantly different from that in females (8.46\u2009\u00b1\u20091.02 nM).<\/jats:p><jats:p>In conclusion, this study suggests that sex differences in fetal sheep lung responses to glucocorticoid therapy are not due to differences in the number or binding affinity of lung GR. Pediatr Pulmonol. 2001; 32:8\u201313. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ppul.1082","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:41:59Z","timestamp":1030311719000},"page":"8-13","source":"Crossref","is-referenced-by-count":17,"title":["Sex differences in response to steroids in preterm sheep lungs are not explained by glucocorticoid receptor number or binding affinity*"],"prefix":"10.1002","volume":"32","author":[{"given":"Jana","family":"Kovar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan J.","family":"Waddell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter D.","family":"Sly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen E.","family":"Willet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,6,14]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1542\/peds.67.5.614","article-title":"Incidence of respiratory distress syndrome following antenatal betamethasone: role of sex, type of delivery, and prolonged rupture of membranes","volume":"67","author":"Papageorgiou ANE","year":"1981","journal-title":"Pediatrics"},{"key":"e_1_2_1_3_2","first-page":"238","article-title":"Discordance between male and female deaths due to the respiratory distress syndrome","volume":"78","author":"Perelman RH","year":"1986","journal-title":"Am J Obstet Gynecol"},{"key":"e_1_2_1_4_2","first-page":"577","volume-title":"Lung growth and development","author":"Horowitz S","year":"1997"},{"key":"e_1_2_1_5_2","first-page":"37","volume-title":"The lung: scientific foundations","author":"Barnes PJ","year":"1997"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(16)32633-3"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199712000-00027"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(00)70231-6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.75.5.2099"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(94)90283-6"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1030031"},{"key":"e_1_2_1_12_2","first-page":"93","volume-title":"Steroid hormones: a practical approach","author":"Leake R","year":"1987"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(82)90002-4"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(84)90371-6"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(80)80204-6"},{"key":"e_1_2_1_16_2","first-page":"456","article-title":"Sex differences in development of fetal rat lung: autoradiographic and biochemical studies","volume":"50","author":"Adamson IYR","year":"1984","journal-title":"Lab Invest"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.3109\/01902148909087851"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.3109\/01902148709068811"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111943"}],"container-title":["Pediatric Pulmonology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fppul.1082","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ppul.1082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T18:47:02Z","timestamp":1700419622000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ppul.1082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6,14]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,7]]}},"alternative-id":["10.1002\/ppul.1082"],"URL":"https:\/\/doi.org\/10.1002\/ppul.1082","archive":["Portico"],"relation":{},"ISSN":["8755-6863","1099-0496"],"issn-type":[{"value":"8755-6863","type":"print"},{"value":"1099-0496","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,6,14]]}}}