{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:08:40Z","timestamp":1770340120942,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,7,24]],"date-time":"2001-07-24T00:00:00Z","timestamp":995932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neutrophil\u2010dominated inflammation is prominent in the cystic fibrosis (CF) and chronic bronchitis (CB) airways. We assessed the degree of airway inflammation by measuring the sputum concentrations of interleukin (IL)\u20108, myeloperoxidase (MPO), and deoxyribonucleic acid (DNA). We determined the relationship among the concentrations of these mediators and investigated methodological problems that may be responsible for reported variability in measurements. Sputa obtained from 31 patients were solubilized with phosphate\u2010buffered saline, dithiothreitol (DTT) (0.1% or 1%), or dornase alfa (0.2 mg\/mL). The sputum concentration of IL\u20108 and MPO was measured by enzyme\u2010linked immunosorbent assay (ELISA), and DNA was measured using microfluorimetry.<\/jats:p><jats:p>There was a significant relationship among sputum IL\u20108, MPO, and DNA. For MPO (means\u2009\u00b1\u2009SD), CF was 1,392\u2009\u00b1\u2009771 vs. CB at 75\u2009\u00b1\u200965 mcg\/mL; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001. For IL\u20108: CF was 239\u2009\u00b1\u2009154 vs. CB at 121\u2009\u00b1\u2009108 ng\/mL; <jats:italic>P<\/jats:italic>\u2009=\u20090.0002. For DNA, CF was 1.707\u2009\u00b1\u20091.25 vs. CB at 0.184\u2009\u00b1\u20090.272 mg\/mL; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001. The MPO concentration in CF sputum was approximately double after in vitro treatment with dornase alfa (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p><jats:p>There is a greater concentration of IL\u20108, MPO, and DNA in CF than in CB sputa. There is a significant relationship among these inflammatory markers in sputum. DNA polymers bind myeloperoxidase in the sputum, and we speculate that treatment with dornase alfa may remove a source of MPO inhibition. Pediatr Pulmonol. 2001; 32:152\u2013158. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ppul.1101","type":"journal-article","created":{"date-parts":[[2004,10,28]],"date-time":"2004-10-28T23:11:04Z","timestamp":1099005064000},"page":"152-158","source":"Crossref","is-referenced-by-count":34,"title":["Sputum processing for evaluation of inflammatory mediators"],"prefix":"10.1002","volume":"32","author":[{"given":"Jung\u2010Soo","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant H.","family":"Hackley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce 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