{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T02:56:04Z","timestamp":1774666564280,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,10,21]],"date-time":"2005-10-21T00:00:00Z","timestamp":1129852800000},"content-version":"vor","delay-in-days":4160,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The diffusing capacity of the lung for carbon monoxide (D<jats:sub>LCO<\/jats:sub>) is an important index of lung function but is not easily measured in spontaneously breathing animals with small lung volumes. Our aim was to devise a simple rebreathing method that would allow us to make serial measurements of D<jats:sub>LCO<\/jats:sub> in spontaneously breathing lambs during their first month after birth. By adding He to the rebreathing gas mixture, we were also able to measure functional residual capacity (FRC), enabling us to normalize D<jats:sub>LCO<\/jats:sub> with respect to FRC. We have compared FRC measured by the rebreathing technique with that measured by a closed\u2010circuit helium dilution method (FRC<jats:sub>cc<\/jats:sub>). Using the rebreathing method we found highly significant positive correlations between D<jats:sub>LCO<\/jats:sub> and body weight (r = 0.70, <jats:italic>P<\/jats:italic> &lt; 0.001) and between FRC and body weight (r = 0.79, <jats:italic>P<\/jats:italic> &lt; 0.001). There was no significant change in D<jats:sub>LCO<\/jats:sub>\/FRC over the first postnatal month; the mean value was 8.1\u00b1 0.6 ML\/min\/mmHg\/mL. Rebreathing FRC was highly correlated with FRC, (r = 0.88, <jats:italic>P<\/jats:italic> &lt; 0.001), but was lower than FRC<jats:sub>cc<\/jats:sub> by about 18%. In normal lambs D<jats:sub>LCO<\/jats:sub> and FRC, but not D<jats:sub>LCO<\/jats:sub>\/FRC, increased during the first month after birth, suggesting that the increase in D<jats:sub>LCO<\/jats:sub> parallels lung growth. We conclude that the modified rebreathing method is suitable for measuring D<jats:sub>LCO<\/jats:sub> in small uncooperative spontaneously breathing animals. <jats:bold>Pediatr Pulmonol. 1994; 17:347\u2013353.<\/jats:bold> \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ppul.1950170602","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:20:09Z","timestamp":1173208809000},"page":"347-353","source":"Crossref","is-referenced-by-count":9,"title":["Measurement of lung diffusing capacity and functional residual capacity in lambs during the first postnatal month"],"prefix":"10.1002","volume":"17","author":[{"given":"David P.","family":"Johns","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra E.","family":"Jakubowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graeme J.","family":"McCrabb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Harding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/136.5.1299"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI103402"},{"key":"e_1_2_1_4_2","first-page":"189","volume-title":"Pulmonary Function Testing: Principles and Practice","author":"Conrad SA","year":"1984"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)92996-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1152\/jappl.1979.46.2.394","article-title":"A system for rapid measurement of lung function in small mammals","volume":"46","author":"Holub D","year":"1979","journal-title":"J Appl Physiol."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197810000-00007"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI102923"},{"key":"e_1_2_1_9_2","first-page":"75","volume-title":"Respiratory Function in Disease","author":"Bates DV","year":"1971"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI103503"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(68)90050-9"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197609000-00001"},{"key":"e_1_2_1_13_2","first-page":"223","volume-title":"The Physiology of the Newborn Infant","author":"Smith CA","year":"1976"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0954-6820.1957.tb00098.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199311000-00011"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1989.67.6.2401"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1915.sp001710"},{"key":"e_1_2_1_18_2","first-page":"87","article-title":"A modified helium dilution technique for measuring small lung volumes","volume":"17","author":"Roy CH","year":"1992","journal-title":"J Dev Physiol."},{"key":"e_1_2_1_19_2","first-page":"327","volume-title":"Normal Values for Respiratory Function in Man","author":"Cotes JE","year":"1970"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.38.3.212"},{"key":"e_1_2_1_21_2","first-page":"537","article-title":"A rebreathing method for measuring carbon monoxide diffusing capacity","volume":"115","author":"Marshall R","year":"1977","journal-title":"Am Rev Resp Dis."},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI103985"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1159\/000241098"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(76)90037-2"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1159\/000194759"},{"key":"e_1_2_1_26_2","doi-asserted-by":"crossref","unstructured":"BerglundG KarlbergP.Determination of the functional residual capacity in newborn infants. 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