{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:24:04Z","timestamp":1762100644007},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,10,24]],"date-time":"2005-10-24T00:00:00Z","timestamp":1130112000000},"content-version":"vor","delay-in-days":4071,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[1994,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leukotriene B<jats:sub>4<\/jats:sub> (LTB<jats:sub>4<\/jats:sub>) and leukotriene C<jats:sub>4<\/jats:sub> (LTC<jats:sub>4<\/jats:sub>) are considered to be important mediators in the pathophysiology of asthma. Theophylline, terbutaline, and hydrocortisone are drugs commonly used in the treatment of asthma. In the present study we have investigated the in vitro inhibitory effects of theophylline, terbutaline, and hydrocortisone on LTB<jats:sub>4<\/jats:sub> and LTC<jats:sub>4<\/jats:sub> generation from human leukocytes. After preincubation in the presence of these drugs, the cells were stimulated with the calcium ionophore A 23187 and the supernatants were analyzed for their LTB, and LTC, content using reverse\u2010phase high\u2010performance liquid chromatography (HPLC). Total leukotriene (LT) production (the combined amounts of LTB<jats:sub>4<\/jats:sub> and LTC<jats:sub>4<\/jats:sub>) was dose\u2010dependently inhibited by pretreatment with theophylline, terbutaline or hydrocortisone. Therapeutic levels of hydrocortisone (5 \u00d7 10<jats:sup>\u22126<\/jats:sup> M) plus theophylline (5 \u00d7 10<jats:sup>\u22125<\/jats:sup> M) inhibited LTB<jats:sub>4<\/jats:sub> and LTC<jats:sub>4<\/jats:sub> production in an additive way, as did the combination of hydrocortisone plus terbutaline (5 \u00d7 10<jats:sup>\u22128<\/jats:sup> M). A statistically significant effect of diminished LTB, generation was obtained after preincubation with therapeutic levels of theophylline plus terbutaline, but no such effect was seen for LTC<jats:sub>4<\/jats:sub> levels. The in vitro inhibitory effects on LTB<jats:sub>4<\/jats:sub> and LTC<jats:sub>4<\/jats:sub> generation from human leukocytes by theophylline, terbutaline, and hydrocortisone, as well as the additive effect of hydrocortisone plus theophylline or terbutaline, add to our understanding of the therapeutic effects of these drugs in the treatment of bronchopulmonary obstruction.<jats:bold>Pediatr Pulmonol. 1994;18:129\u2013134.<\/jats:bold> \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ppul.1950180302","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:39:36Z","timestamp":1173209976000},"page":"129-134","source":"Crossref","is-referenced-by-count":5,"title":["Inhibitory effects of theophylline, terbutaline, and hydrocortisone on leukotriene B<sub>4<\/sub> and C<sub>4<\/sub> generation by human leukocytes in vitro"],"prefix":"10.1002","volume":"18","author":[{"given":"T.","family":"Shimizu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kristj\u00e1nsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Wennergren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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