{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:06:53Z","timestamp":1758816413676},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,6,16]],"date-time":"2004-06-16T00:00:00Z","timestamp":1087344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Eosinophils play an important role in inflammation and probably in airway remodeling in asthma. We previously demonstrated that eosinophils from atopic subjects display pro\u2010fibrogenic properties towards lung fibroblasts partially by preformed transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2). We hypothesized that the pro\u2010fibrogenic potential of eosinophils is increased in children with life\u2010threatening asthma (LTA). Six children with atopic LTA clinically well\u2010controlled by inhaled corticosteroids (ICS) and 5 children with atopic mild asthma (MA) treated only with inhaled \u03b2<jats:sub>2<\/jats:sub>\u2010agonists were investigated. The effects of their peripheral blood eosinophils on fibroblast proliferation and lattice contraction were investigated. In addition, TGF\u2010\u03b2<jats:sub>1<\/jats:sub> and IL\u20106 eosinophil content were also evaluated. Unexpectedly, eosinophils from LTA increased fibroblast proliferation (5.4\u2010fold) and gel contraction (1.1\u2010fold) significantly less than those from MA. TGF\u2010\u03b2<jats:sub>1<\/jats:sub> but not IL\u20106 eosinophil content in LTA was significantly lower than that in MA (2.7\u2010fold). In vitro, addition of dexamethasone on eosinophils stimulated by mast cells resulted in a marked decrease in their TGF\u2010\u03b2<jats:sub>1<\/jats:sub> content by 1.6\u2010fold. In conclusion, eosinophils from children with ICS\u2010treated LTA displayed significantly less pro\u2010fibrogenic properties than those from MA treated only with \u03b2<jats:sub>2<\/jats:sub>\u2010agonists. Our data suggest that the pro\u2010fibrogenic effect of eosinophils might be influenced by treatment with ICS in childhood asthma. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ppul.20067","type":"journal-article","created":{"date-parts":[[2004,6,16]],"date-time":"2004-06-16T20:01:00Z","timestamp":1087416060000},"page":"222-228","source":"Crossref","is-referenced-by-count":5,"title":["Reduced eosinophil pro\u2010fibrogenic effect in severe childhood asthma compared to mild disease: An effect of corticosteroids?"],"prefix":"10.1002","volume":"38","author":[{"given":"Ilaria","family":"Puxeddu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gideon","family":"Lack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan 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