{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:42:16Z","timestamp":1788871336721,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,2,27]],"date-time":"2008-02-27T00:00:00Z","timestamp":1204070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Acad\u00e9mie Nationale de M\u00e9decine, France"},{"name":"Fondation de l'Avenir, France"},{"name":"SmithKline Beecham Institut, 92731 Nanterre, France"},{"name":"Centre Hospitalier Universitaire Vaudois (CHUV), Service de Chirurgie P\u00e9diatrique, Lausanne, Switzerland"},{"name":"Fond Decker, CHUV, Switzerland"},{"name":"Fidax S.A., Switzerland"},{"name":"Leo Pharmaceutical Products Copenhagen, Denmark"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The aim of this study was to determine the presence of the neuronal nitric oxide synthase (nNOS) in near full\u2010term lambs with congenital diaphragmatic hernia (CDH) and its role in the modulation of pulmonary vascular basal tone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We surgically created diaphragmatic hernia on the 85th day of gestation. On the 135th, catheters were used to measure pulmonary pressure and blood flow. We tested the effects of 7\u2010nitroindazole (7\u2010NINA), a specific nNOS antagonist and of\n                      <jats:italic>N<\/jats:italic>\n                      \u2010nitro\u2010\n                      <jats:sc>l<\/jats:sc>\n                      \u2010arginine (\n                      <jats:sc>l<\/jats:sc>\n                      \u2010NNA), a nonspecific nitric oxide synthase antagonist. In vitro, we tested the effects of the same drugs on isolated pulmonary vessels. The presence of nNOS protein in the lungs was detected by Western blot analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Neither 7\u2010NINA nor\n                      <jats:sc>l<\/jats:sc>\n                      \u2010NNA modified pulmonary vascular basal tone in vivo. After\n                      <jats:sc>l<\/jats:sc>\n                      \u2010NNA injection, acetylcholine (ACh) did not decrease significantly pulmonary vascular resistance (PVR). In vitro,\n                      <jats:sc>l<\/jats:sc>\n                      \u2010NNA increased the cholinergic contractile\u2010response elicited by electric field stimulation (EFS) of vascular rings from lambs with diaphragmatic hernia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We conclude that nNOS protein is present in the lungs and pulmonary artery of near full\u2010term lamb fetuses with diaphragmatic hernia, but that it does not contribute to the reduction of pulmonary vascular tone at birth. Pediatr Pulmonol. 2008; 43:313\u2013321. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ppul.20796","type":"journal-article","created":{"date-parts":[[2008,2,27]],"date-time":"2008-02-27T16:47:25Z","timestamp":1204130845000},"page":"313-321","source":"Crossref","is-referenced-by-count":3,"title":["Neuronal nitric oxide synthase does not contribute to the modulation of pulmonary vascular tone in fetal lambs with congenital diaphragmatic hernia (nNOS in CDH lambs)"],"prefix":"10.1002","volume":"43","author":[{"given":"Anthony S. de Buys","family":"Roessingh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"de 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