{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:31:15Z","timestamp":1773419475183,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T00:00:00Z","timestamp":1265155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Maternal smoking during pregnancy increases the incidence and severity of respiratory infections in neonates. Surfactant proteins A and D (SP\u2010A and SP\u2010D, respectively) are components of pulmonary innate immunity and have an important role in defense against inhaled pathogens. The purpose of this study was to determine if nicotine exposure during the third trimester of pregnancy alters the expression of SP\u2010A and SP\u2010D of fetal lung epithelia. Pregnant ewes were assigned to four groups; a nicotine\u2010exposed full\u2010term and pre\u2010term group, and control full\u2010term and pre\u2010term group. Lung tissue was collected for Western blot and IHC analysis of SP\u2010A level, Western blot analysis of SP\u2010D level and qPCR analysis of SP\u2010A and SP\u2010D mRNA expression. Exposure to nicotine significantly decreased SP\u2010A gene expression (<jats:italic>P<\/jats:italic>\u2009=\u20090.01) and SP\u2010A protein level in pre\u2010term lambs. This finding suggests that maternal nicotine exposure during the last trimester of pregnancy alters a key component of lung innate immunity in offspring. Pediatr Pulmonol. 2010; 45:255\u2013262. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ppul.21153","type":"journal-article","created":{"date-parts":[[2010,2,3]],"date-time":"2010-02-03T18:52:13Z","timestamp":1265223133000},"page":"255-262","source":"Crossref","is-referenced-by-count":10,"title":["Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia"],"prefix":"10.1002","volume":"45","author":[{"given":"Tatjana","family":"Lazic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Matic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack M.","family":"Gallup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Van Geelen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David 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