{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T07:14:28Z","timestamp":1696922068980},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,5,29]],"date-time":"2012-05-29T00:00:00Z","timestamp":1338249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study investigated environmental endotoxin exposure during early life, sensitization to aeroallergens, the production of cytokines by LPS\u2010stimulated leukocytes, and the development of a wheezing phenotype in a prospective cohort of infants with high risk of developing allergic diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Eighty\u2010four infants were followed from birth until 30 months of age. We assessed endotoxin concentration in house dust of their homes during the first 6 months of life. At age 30 months they were clinically evaluated to determine the development of wheezing and other clinical events, were skin prick tested, and had blood samples collected for the evaluation of cytokine release by LPS\u2010stimulated peripheral blood mononuclear cells (PBMC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The level of endotoxin exposure during early life was not associated with development of a wheezing phenotype. On the other hand a higher incidence of respiratory infections occurred among recurrent wheezing (RW) infants. PBMC from RW children exposed to higher levels of environmental endotoxin (above 50\u2009EU\/mg) released less Interleukin (IL)\u201012p70 and IFN\u2010\u03b3 compared to the non\u2010RW group. TNF\u2010\u03b1, IL\u201010, IL\u20104, IL\u20105, and IL17 production by LPS\u2010stimulated PBMC from RW and non\u2010RW children was equivalent in both groups of environmental endotoxin exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this prospective cohort of infants with high risk of developing allergic diseases we observed that RW and non\u2010RW children were exposed to similar levels of endotoxin early in life. LPS\u2010stimulated PBMC from RW infants exposed to higher levels of endotoxin released significantly less IL\u201012 and IFN\u2010\u03b3 compared to non\u2010RW infants. Pediatr Pulmonol. 2012. 47:1054\u20131060. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.22598","type":"journal-article","created":{"date-parts":[[2012,5,29]],"date-time":"2012-05-29T12:29:30Z","timestamp":1338294570000},"page":"1054-1060","source":"Crossref","is-referenced-by-count":1,"title":["Leukocytes from wheezing infants release lower amounts of IL\u201012 and IFN\u2010\u03b3 compared to non\u2010wheezing infants"],"prefix":"10.1002","volume":"47","author":[{"given":"Angela","family":"Falcai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo V\u00edtor Soeiro","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina Arslanian","family":"Kubo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"Rullo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo Rugero","family":"Errante","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirceu","family":"Sol\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Condino\u2010Neto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,29]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1806-37132008000600001"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199501193320301"},{"key":"e_1_2_5_4_2","first-page":"516","article-title":"Wheezing conditions in early childhood: prevalence and risk factors in the city of Sao Paulo, Brazil","volume":"82","author":"Benicio MH","year":"2004","journal-title":"Bull World Health Organ"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200504-525OC"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1157\/13080928"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000218120"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00032-09"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2009.04.011"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1526-0542(03)00052-6"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3016.2007.00878.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00959.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02239-X"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03424.x"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.2.2002088"},{"key":"e_1_2_5_16_2","first-page":"225","article-title":"Exposure to house dust endotoxin and allergic sensitization in allergic and nonallergic children living in Adana, Turkey","volume":"51","author":"Yilmaz M","year":"2009","journal-title":"Turk J Pediatr"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00002108"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02175.x"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1991.tb01683.x"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1157\/13115665"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/09629350152700966"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.1.86"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.03.037"},{"key":"e_1_2_5_24_2","first-page":"28","article-title":"Selective deficiency of interferon\u2010gamma production in the hyper\u2010IgE syndrome. 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