{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:16:35Z","timestamp":1790619395212,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T00:00:00Z","timestamp":1507852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006181","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado da Bahia","doi-asserted-by":"publisher","award":["PPSUS No. SUS0023\/2013"],"award-info":[{"award-number":["PPSUS No. SUS0023\/2013"]}],"id":[{"id":"10.13039\/501100006181","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9liuksen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To assess the role of human bocavirus 1 (HBoV1) as a causative agent of non\u2010severe community\u2010acquired pneumonia (CAP) in children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients aged 2\u201059 months with non\u2010severe CAP (respiratory complaints and radiographic pulmonary infiltrate\/consolidation) attending a University Hospital in Salvador, Brazil were enrolled in a prospective cohort. From 820 recruited children in a clinical trial (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/ClinicalTrials.gov\">ClinicalTrials.gov<\/jats:ext-link> NCT01200706), nasopharyngeal aspirate (NPA), and acute and convalescent serum samples were obtained from 759 (92.6%) patients. NPAs were tested for 16 respiratory viruses by PCR. Acute HBoV1 infection was confirmed by measuring specific IgM and IgG responses in paired serum samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Respiratory viruses were detected in 693 (91.3%; 95%CI: 89.1\u201093.2) CAP cases by PCR. HBoV1\u2010DNA was detected in 159 (20.9%; 95%CI: 18.2\u201024.0) cases. Of these 159 PCR positive cases, acute HBoV1 infection was confirmed serologically in 38 cases (23.9%; 95%CI: 17.8\u201031.0). Overall, acute HBoV1 infection was confirmed in 5.0% (38\/759) of non\u2010severe CAP patients. HBoV1 was detected in 151 cases with at least one other virus making 31.7% of all multiple virus (<jats:italic>n<\/jats:italic>\u2009=\u2009477) detections. Among all 759 cases, 216 had one respiratory virus detected, and sole HBoV1 was detected in only 8 (3.7%). Acute HBoV1 infection was serologically diagnosed in 34 (22.5%) HBoV1\u2010DNA\u2010positive cases with another virus, compared to 4 (50.0%) cases with sole virus detection (<jats:italic>p<\/jats:italic>\u2009=\u20090.09).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HBoV1 was detected by PCR in one fifth of the children with non\u2010severe CAP and acute HBoV1 infection was serologically confirmed in one quarter of these cases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.23891","type":"journal-article","created":{"date-parts":[[2017,10,13]],"date-time":"2017-10-13T14:02:54Z","timestamp":1507903374000},"page":"88-94","source":"Crossref","is-referenced-by-count":18,"title":["Serologically diagnosed acute human bocavirus 1 infection in childhood community\u2010acquired pneumonia"],"prefix":"10.1002","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9678-0771","authenticated-orcid":false,"given":"Amanda C.","family":"Nascimento\u2010Carvalho","sequence":"first","affiliation":[{"name":"Bahiana School of Medicine Bahiana Foundation for Science Development Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u2010Luisa","family":"Vilas\u2010Boas","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Federal University of Bahia School of Medicine Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria\u2010Socorro H.","family":"Fontoura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Federal University of Bahia School of Medicine Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Virology University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tytti","family":"Vuorinen","sequence":"additional","affiliation":[{"name":"Department of Clinical Virology Turku University Hospital and Department of Virology University of Turku Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"S\u00f6derlund\u2010Venermo","sequence":"additional","affiliation":[{"name":"Department of Virology University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olli","family":"Ruuskanen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Turku Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana M.","family":"Nascimento\u2010Carvalho","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Federal University of Bahia School of Medicine Salvador Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the PNEUMOPAC\u2010Efficacy Study Group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60222-6"},{"key":"e_1_2_7_3_1","first-page":"196","article-title":"Community acquired pneumonia","volume":"77","author":"Polverino E","year":"2011","journal-title":"Minerva Anestesiol"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23438"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61459-6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"12801","DOI":"10.1073\/pnas.0504666102","article-title":"Cloning of a human parvovirus by molecular screening of respiratory tract samples","volume":"102","author":"Allander T","year":"2005","journal-title":"Proc Natl Acad Sci"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/512196"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1205.051523"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00889-06"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/526532"},{"key":"e_1_2_7_11_1","unstructured":"World Health Organization. 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