{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:02:26Z","timestamp":1774321346837,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,14]],"date-time":"2017-11-14T00:00:00Z","timestamp":1510617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess whether children \u22645 years of age, produce sufficient amounts of interferon gamma (IFN\u2010\u0263) in response to phytohaemagglutinin (mitogen), and <jats:italic>Mycobacterium tuberculosis<\/jats:italic> antigens (TB antigens) in the QuantiFERON\u2010TB Gold in\u2010Tube test (QFT\u2010GIT), (Cellestis Ltd., Australia). Working hypothesis: Is TB\u2010antigen\u2010induced IFN\u2010\u0263 response in children \u22645 years sufficient to consider QFT\u2010GIT a possible tool for TB diagnostics? Study design, patient\u2010subject selection, and methods: We recruited children 0\u201017 years old suspected of TB infection to this cross\u2010sectional study, in whom QFT\u2013GIT and TST were performed. We analyzed the median IFN\u2010\u0263 levels in mitogen and TB antigen tubes in children \u22645 years and &gt;5 years, and the correlation between IFN\u2010\u0263 level in both tubes and age.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 153 children were enrolled, age median was 7.8 (IQR:8), 45 (29.4%) aged \u22645 years (median 3.4, IQR:1.7), 108\u2009&gt;\u20095 years (median 10.55, IQR:5.93). In the mitogen tubes, the median IFN\u2010\u0263 level was higher in children &gt;5 years (median 17.87, IQR:2.1 vs 16.77, IQR:7.6), but surprisingly in the TB antigen tubes it was higher in the younger group (median 0.12, IQR:0.21vs 0.06, IQR:0.09, <jats:italic>P<\/jats:italic>\u2009=\u20090.04). We proved a positive correlation between IFN\u2010\u0263 level and age in mitogen tubes (<jats:italic>r<\/jats:italic>\u2009=\u20090.18, <jats:italic>P<\/jats:italic>\u2009=\u20090.03) and a negative correlation in TB antigen tubes (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.17, <jats:italic>P<\/jats:italic>\u2009=\u20090.04). In latent tuberculosis infection patients, the latter correlation was found to be even stronger (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.39, <jats:italic>P<\/jats:italic>\u2009=\u20090.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The youngest children release sufficient amount of IFN\u2010\u0263 in response to TB antigens thus QFT\u2010GIT might be a useful tool for TB diagnostics in this age group.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.23910","type":"journal-article","created":{"date-parts":[[2017,11,14]],"date-time":"2017-11-14T15:06:40Z","timestamp":1510672000000},"page":"181-188","source":"Crossref","is-referenced-by-count":5,"title":["Is secretion of IFN\u2010gamma in response to <i>Mycobacterium tuberculosis<\/i> antigens in youngest children sufficient to play a role in TB diagnostics?"],"prefix":"10.1002","volume":"53","author":[{"given":"Teresa","family":"Bielecka","sequence":"first","affiliation":[{"name":"Department of Pediatric Pulmonology and Allergy Medical University of Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5034-538X","authenticated-orcid":false,"given":"Anna","family":"Komorowska\u2010Piotrowska","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology and Allergy Medical University of Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Krenke","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology and Allergy Medical University of Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wojciech","family":"Feleszko","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology and Allergy Medical University of Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Kulus","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology and Allergy Medical University of Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30474-1"},{"key":"e_1_2_8_3_1","unstructured":"World Health Organization. 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