{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T19:24:45Z","timestamp":1777577085325,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T00:00:00Z","timestamp":1510099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1S10OD011925\u201001"],"award-info":[{"award-number":["1S10OD011925\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R03PA13\u2010304"],"award-info":[{"award-number":["R03PA13\u2010304"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1T32AI089443"],"award-info":[{"award-number":["1T32AI089443"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infections worldwide, causing disproportionate morbidity and mortality in infants and children. Infants with stronger Th1 responses have less severe disease, yet little is known about the infant T\u2010cell response within the air space. Thus, we tested the hypothesis that RSV infected infant mice would have quantitative and qualitative deficiencies in CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cell populations isolated from the bronchoalveolar lavage when compared to adults and that local delivery of IFN\u2010\u03b3 would increase airway CD4<jats:sup>+<\/jats:sup> Tbet<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> Tbet<jats:sup>+<\/jats:sup> T\u2010cell responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared the localization of T\u2010cell responses in RSV\u2010infected infant and adult mice and investigated the effects of local IFN\u2010\u03b3 administration on infant cellular immunity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Adult CD8<jats:sup>+<\/jats:sup> CD44<jats:sup>HI<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> CD44<jats:sup>HI<\/jats:sup> Tbet<jats:sup>+<\/jats:sup> T\u2010cells accumulated in the alveolar space whereas CD4<jats:sup>+<\/jats:sup> CD44<jats:sup>HI<\/jats:sup> Tbet<jats:sup>+<\/jats:sup> T\u2010cells were evenly distributed between the infant lung tissue and airway and infant lungs contained higher frequencies of CD8<jats:sup>+<\/jats:sup> T\u2010cells. Delivery of IFN\u2010\u03b3 to the infant airway failed to increase the accumulation of T\u2010cells in the airspace and unexpectedly reduced CD4<jats:sup>+<\/jats:sup> CD44<jats:sup>HI<\/jats:sup> Tbet<jats:sup>+<\/jats:sup> T\u2010cells. However, intranasal IFN\u2010\u03b3 increased RSV F protein\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cells in the alveolar space.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Together, these data suggest that quantitative and qualitative defects exist in the infant T\u2010cell response to RSV but early, local IFN\u2010\u03b3 exposure can increase the CD8<jats:sup>+<\/jats:sup> RSV\u2010specific T\u2010cell response.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.23911","type":"journal-article","created":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T09:28:45Z","timestamp":1510133325000},"page":"145-153","source":"Crossref","is-referenced-by-count":14,"title":["Localization of the T\u2010cell response to RSV infection is altered in infant mice"],"prefix":"10.1002","volume":"53","author":[{"given":"Katherine M.","family":"Eichinger","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Therapeutics University of Pittsburgh School of Pharmacy, University of Pittsburgh Pittsburgh Pennsylvania"},{"name":"Center for Clinical Pharmaceutical Sciences University of Pittsburgh School of Pharmacy, University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica L.","family":"Kosanovich","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Therapeutics University of Pittsburgh School of Pharmacy, University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7531-2541","authenticated-orcid":false,"given":"Kerry M.","family":"Empey","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Therapeutics University of Pittsburgh School of Pharmacy, University of Pittsburgh Pittsburgh Pennsylvania"},{"name":"Center for Clinical Pharmaceutical Sciences University of Pittsburgh School of Pharmacy, University of Pittsburgh Pittsburgh Pennsylvania"},{"name":"Department of Immunology University of Pittsburgh School of Medicine University of Pittsburgh Pittsburgh Pennsylvania"},{"name":"Clinical and Translational Science Institute University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030076"},{"key":"e_1_2_7_3_1","volume-title":"Global Health Estimates 2015: Deaths by Cause, Age, Sex, by Country and by Region, 2000\u20132015","year":"2016"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60206-1"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40266-015-0258-9"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40121-014-0046-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2013-0303"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0804877"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.3.1163"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115362"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01770-12"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200209)32:9<2562::AID-IMMU2562>3.0.CO;2-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198607103150201"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/modpathol.3800725","article-title":"The histopathology of fatal untreated human respiratory syncytial virus infection","volume":"20","author":"Johnson JE","year":"2006","journal-title":"Mod Pathol"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/512615"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.71.5.428"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.12.8410"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903075"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.07.012"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1086\/518249"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200210-1148OC"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830271233"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.7.3698"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00388-1"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040499"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890260207"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5704"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169273"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02313-07"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI72948"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1110203109"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.11.5654-5666.2002"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2006.04.023"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0102-54"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200208)32:8<2117::AID-IMMU2117>3.0.CO;2-C"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00185-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-015-0282-7"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200612-1890OC"},{"key":"e_1_2_7_39_1","doi-asserted-by":"crossref","first-page":"735","DOI":"10.4049\/jimmunol.176.2.735","article-title":"Cutting edge: innate immune cells contribute to the IFN\u2010\u03b3\u2010Dependent regulation of antigen\u2010Specific CD8+ T cell homeostasis","volume":"176","author":"\u00d6 Sercan","year":"2006","journal-title":"J Immunol"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902708"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402058"}],"container-title":["Pediatric Pulmonology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fppul.23911","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ppul.23911","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T09:41:56Z","timestamp":1695894116000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ppul.23911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,8]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/ppul.23911"],"URL":"https:\/\/doi.org\/10.1002\/ppul.23911","archive":["Portico"],"relation":{},"ISSN":["8755-6863","1099-0496"],"issn-type":[{"value":"8755-6863","type":"print"},{"value":"1099-0496","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,8]]}}}