{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:48:54Z","timestamp":1790797734613,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,5,22]],"date-time":"2019-05-22T00:00:00Z","timestamp":1558483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672062"],"award-info":[{"award-number":["81672062"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7152025"],"award-info":[{"award-number":["7152025"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To analyze the molecular and the clinical characteristics of <jats:italic>Mycoplasma pneumoniae<\/jats:italic> (Mp) pneumonia (MPP) and to explore the related factors predicting severe MPP (SMPP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 423 pediatric cases of MPP were retrospectively analyzed, in 2013\u20102017, in Beijing, China. Clinical information was collected from the medical records. Mp\u2010positive specimens were characterized using P1 typing and multiple locus variable\u2010number tandem repeat analysis (MLVA). The macrolide resistance\u2010associated mutations were also detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The predominant genotype was P1\u20101 (88.2%) and M4\u20105\u20107\u20102 (87.5%), whereas percentages of type P1\u20102 and M3\u20105\u20106\u20102 increased across the 5\u2010year period. The mutation rate of genotype M4\u20105\u20107\u20102 (365\/370, 98.6%) was significantly higher than that of the genotype M3\u20105\u20106\u20102 (15\/48, 32.25%; <jats:italic>P<\/jats:italic>\u2009=\u20090.000). Overall, 180 (42.6%) of the 423 Mp\u2010positive patients were coinfected with other pathogens. Respiratory syncytial virus coinfection (24\/180, 13.3%) was more common in cases typed M3\u20105\u20106\u20102 (4\/23, 17.4%) than that of M4\u20105\u20107\u20102 (20\/155, 12.9%; <jats:italic>P<\/jats:italic>\u2009=\u20090.038). Pleural effusion accounted for 52.6% (169\/321) of the observed complications. In the mono\u2010infection cases, cases typed M3\u20105\u20106\u20102 (56%, 14\/25) were significantly (<jats:italic>P<\/jats:italic>\u2009=\u20090.020) associated with pleural effusion compared with those typed M4\u20105\u20107\u20102 (32.6%, 70\/215); 84% (21\/25) of specimens typed M3\u20105\u20106\u20102 were diagnosed as SMPP, whereas 63.7% (137\/215) of specimens typed M4\u20105\u20107\u20102 were diagnosed as SMPP (<jats:italic>P<\/jats:italic>\u2009=\u20090.043).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In our study, we proposed for the first time that the mono\u2010infection patients with Mp typed M3\u20105\u20106\u20102 appear to have a higher risk for progressing to SMPP. MLVA typing can provide hints on the clinical characteristics of Mpp.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.24327","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T14:32:08Z","timestamp":1558621928000},"page":"1012-1021","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":72,"title":["Molecular and clinical characteristics of severe <i>Mycoplasma pneumoniae<\/i> pneumonia in children"],"prefix":"10.1002","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5624-8636","authenticated-orcid":false,"given":"Chao","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Bacteriology Capital Institute of Pediatrics Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanhua","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Bacteriology Capital Institute of Pediatrics Beijing 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