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Adolescents without asthma, who had elevated FeNO (FeNO<jats:sub>100<\/jats:sub>\u2009&gt;\u200915 ppb) (n\u2009=\u200919), and control subjects, with low FeNO (FeNO<jats:sub>100<\/jats:sub>\u2009&lt;\u20095 ppb) and without reported symptoms of asthma or allergy (n\u2009=\u200928), participated in a follow\u2010up study where FeNO<jats:sub>50<\/jats:sub>, airway responsiveness to methacholine (PD<jats:sub>20<\/jats:sub>), blood eosinophil counts, and serum neutrophil lipocalin (HNL) and myeloperoxidase (MPO) levels were measured. Questionnaire follow\u2010ups were performed 4 and 16 years later.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Airway responsiveness (PD<jats:sub>20<\/jats:sub>: 6.94 [1.87, 11.39] vs 11.42 [6.33, 59.4]\u2009\u00b5mol; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05) and blood eosinophil counts (0.31 [0.20, 0.44] vs 0.13 [0.1, 0.22]\u200910<jats:sup>9<\/jats:sup>\/L; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001) (geometric mean [95% CI]) were higher among cases than controls. A significant correlation between blood eosinophils and FeNO was found (rho\u2009=\u20090.41; <jats:italic>P<\/jats:italic>\u2009=\u2009.005). In contrast, serum HNL and MPO were lower in cases than controls (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05 both), and there was a negative correlation between HNL and FeNO (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.31; <jats:italic>P<\/jats:italic>\u2009=\u2009.04). At both follow\u2010ups, a higher proportion of subjects reported allergic symptoms compared with baseline (<jats:italic>P<\/jats:italic>\u2009=\u2009.02, <jats:italic>P<\/jats:italic>\u2009=\u2009.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Elevated FeNO in nonasthmatic adolescents was associated with airway hyperresponsiveness, elevated blood eosinophil counts, and lower systemic activation of neutrophils.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.24578","type":"journal-article","created":{"date-parts":[[2020,1,15]],"date-time":"2020-01-15T17:39:33Z","timestamp":1579109973000},"page":"571-579","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Concurrence of elevated FeNO and airway hyperresponsiveness in nonasthmatic adolescents"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5180-5026","authenticated-orcid":false,"given":"Pia","family":"Kalm\u2010Stephens","sequence":"first","affiliation":[{"name":"Department of Women's and Children's Health Uppsala University Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrei","family":"Malinovschi","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences: Clinical Physiology Uppsala University Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christer","family":"Janson","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences: Respiratory, Allergy, and Sleep Research Uppsala University Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Venge","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences: Clinical Chemistry Uppsala University Uppsala 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