{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T20:01:58Z","timestamp":1773777718765,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Project funded by China Postdoctoral Science Foundation","award":["Postdoc no. 241006"],"award-info":[{"award-number":["Postdoc no. 241006"]}]},{"name":"Science and Technology Planning Project 2019 of the Bureau of Science and Technology of Foshan City","award":["1920001000338"],"award-info":[{"award-number":["1920001000338"]}]},{"DOI":"10.13039\/501100012245","name":"Science and Technology Planning Project of Guangdong Province","doi-asserted-by":"crossref","award":["2014A020212025"],"award-info":[{"award-number":["2014A020212025"]}],"id":[{"id":"10.13039\/501100012245","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lung hypoplasia is the main cause of congenital diaphragmatic hernia (CDH)\u2010associated death but pathogenesis remains unclear. MiR\u2010455\u20105p is involved in lung hypoplasia. We hypothesized that nitrofen causes abnormal miR\u2010455\u20105p expression during lung development and designed this study to determine the relationship between miR\u2010455\u20105p, stimulated by retinoic acid 6 (<jats:italic>STRA6<\/jats:italic>), and retinol in a nitrofen\u2010induced CDH with lung hypoplasia rat model. Nitrofen or olive oil was administered to Sprague\u2010Dawley rats by gavage on day 9.5 of gestation, and the rats were divided into a nitrofen group and a control group (n\u2009=\u20096). The left lung of fetuses was dissected on day 15.5. The expression of miR\u2010455\u20105p or <jats:italic>STRA6<\/jats:italic> messenger RNA (mRNA) was determined by quantitative real\u2010time polymerase chain reaction. Average integrated optical density (IOD) of <jats:italic>STRA6<\/jats:italic> protein was determined by immunofluorescence histochemistry. The average retinol level was detected by enzyme\u2010linked immunosorbent assay (n\u2009=\u20096 lungs, respectively). Compared with the control group, the nitrofen group exhibited significantly increased miR\u2010455\u20105p expression levels (29.450\u2009\u00b1\u20099.253 vs 5.955\u2009\u00b1\u20092.330; <jats:italic>P<\/jats:italic>\u2009=\u2009.00045) and significantly decreased <jats:italic>STRA6<\/jats:italic> mRNA levels (0.197\u2009\u00b1\u20090.097 vs 0.588\u2009\u00b1\u20090.184; <jats:italic>P<\/jats:italic>\u2009=\u2009.0047). In addition, the average IOD of the <jats:italic>STRA6<\/jats:italic> protein was significantly lower in the nitrofen group (805.643\u2009\u00b1\u2009291.182 vs 1616.391\u2009\u00b1\u2009572.308, <jats:italic>P<\/jats:italic>\u2009=\u2009.015), and the average retinol level was significantly reduced (4.013\u2009\u00b1\u20090.195 vs 5.317\u2009\u00b1\u20090.337\u2009\u00b5g\/L, <jats:italic>P<\/jats:italic>\u2009=\u2009.000). In summary, the overexpression of miR\u2010455\u20105p affected retinol absorption by downregulating <jats:italic>STRA6<\/jats:italic> in the nitrofen\u2010induced CDH with lung hypoplasia rat model, and this downregulation may be one cause of CDH with lung hypoplasia.<\/jats:p>","DOI":"10.1002\/ppul.24739","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T17:55:59Z","timestamp":1585763759000},"page":"1433-1439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Overexpression of miR\u2010455\u20105p affects retinol (vitamin A) absorption by downregulating <i>STRA6<\/i> in a nitrofen\u2010induced CDH with lung hypoplasia rat model"],"prefix":"10.1002","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0667-0067","authenticated-orcid":false,"given":"Jintao","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Neonatal and Pediatric Surgery Foshan Women and Children Hospital Affiliated to Southern Medical University Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuming","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center Guangzhou Medical University Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huajian","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Neonatal and Pediatric Surgery Foshan Women and Children Hospital Affiliated to Southern Medical University Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiequan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neonatal and Pediatric Surgery Foshan Women and Children Hospital Affiliated to Southern Medical University Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiyu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Neonatal and Pediatric Surgery Foshan Women and Children Hospital Affiliated to Southern Medical University Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangming","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Urology, Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neonatal and Pediatric Surgery Foshan Women and Children Hospital Affiliated to Southern Medical University Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2016.02.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20230"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0009922812472249"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2001.20032"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2007.01.008"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2007.22.4.687"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-7-1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003830050550"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(99)90010-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00108.2014"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0032-1329409"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-016-3886-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1136244"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes5030425"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/512203"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24145"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2014.1796"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0368OC"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1086\/518177"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.33038"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.22.010402.102745E"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00257.2012"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-002-0865-5"},{"issue":"2","key":"e_1_2_7_27_1","first-page":"L423","article-title":"Vitamin 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