{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T06:31:15Z","timestamp":1778999475243,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,6,7]],"date-time":"2020-06-07T00:00:00Z","timestamp":1591488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81301392"],"award-info":[{"award-number":["81301392"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Streptococcus pneumoniae<\/jats:italic> is a major cause of bacterial infection among infants and young children with high morbidity and mortality. The serotype distribution of <jats:italic>S. pneumoniae<\/jats:italic> varies with geography, time, age, and disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>We aimed to investigate the current status of molecular characteristics of <jats:italic>S. pneumoniae<\/jats:italic> strains isolated from pediatric patients in Shanghai, China.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Between 2016 and 2018, 73 clinical <jats:italic>S. pneumoniae<\/jats:italic> isolates were characterized by capsular serotype, multilocus sequence typing, antibiotic susceptibility, and resistant genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The most common serotypes were 19F (39.7%), 19A (16.4%), 6A (11.0%), 14 (9.6%), and 6B (8.2%). The coverage rates of the 7\u2010, 10\u2010 and 13\u2010valent pneumococcal conjugate vaccines were 64.4%, 64.4%, and 91.8%, respectively. The five predominant sequence types were ST271 (37.0%), ST320 (19.2%), ST3173 (11.0%), ST876 (6.8%), and ST81 (4.1%), which were mainly associated with serotypes 19F, 19A, 6A, 14, and 23F, respectively. The rates of resistance to penicillin and ceftriaxone were 21.9% and 39.7%, respectively. All strains displayed resistance to macrolides, 54.8% of which possessed both erm(B) and mef(A\/E) genes, and 41.1% carried the erm(B) gene alone. Tn2010 (41.1%) was the most common transposon.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Clonal complex 271 (Taiwan19F\u201014 clone) played a dominant role in the dissemination of pneumococcal isolates. The prevalent serotypes indicated a lack of the 7\u2010valent pneumococcal conjugate vaccine, which has not been included in national immunization programs in mainland China. The high rate of macrolide resistance made the empirical use of macrolides alone not suitable for treating pediatric pneumococcal disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.24877","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T17:38:19Z","timestamp":1590773899000},"page":"2135-2141","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Molecular characterization of <i>Streptococcus pneumoniae<\/i> isolated from pediatric patients in Shanghai, China"],"prefix":"10.1002","volume":"55","author":[{"given":"Xing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Shanghai Children's Medical Center Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhilei","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Emergency, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weichun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Shanghai Children's Medical Center Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7912-3297","authenticated-orcid":false,"given":"Cong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61204-6"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1000348"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05658-11"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03344-14"},{"key":"e_1_2_6_6_1","first-page":"93","article-title":"Pneumococcal conjugate vaccine for childhood immunization\u2013WHO position paper","volume":"82","author":"WHO","year":"2007","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.601-607.2003"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.124-131.2006"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00484-09"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"issue":"7","key":"e_1_2_6_11_1","first-page":"576","article-title":"E\u2010test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms","volume":"63","author":"Periodontol J.","year":"1992","journal-title":"Nachnani S1"},{"key":"e_1_2_6_12_1","first-page":"M100","volume-title":"Performance Standards for Antimicrobial Disk Susceptibility Tests","author":"Clinical and Laboratory Standards Institute","year":"2017"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-11-3049"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.11.2562"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3313-3318.2002"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.2979-2984.2000"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01384-06"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.3.790-793.2000"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.6.763"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2009.05.010"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.05.026"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067507"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-228"},{"issue":"11","key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"e0142892","DOI":"10.1371\/journal.pone.0142892","article-title":"Serotype distribution, antimicrobial susceptibility, and molecular epidemiology of Streptococcus pneumoniae isolated from children in Shanghai, China","volume":"10","author":"Fen P","year":"2015","journal-title":"PLoS One"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00258"},{"key":"e_1_2_6_26_1","unstructured":"SongSF. 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