{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T05:00:57Z","timestamp":1777352457487,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000222","name":"Heart and Stroke Foundation of Canada","doi-asserted-by":"publisher","award":["G190024330"],"award-info":[{"award-number":["G190024330"]}],"id":[{"id":"10.13039\/501100000222","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Rationale<\/jats:title><jats:p>Point\u2010of\u2010care ultrasound (POCUS) is used to evaluate pulmonary edema in adults with acute respiratory distress syndrome (ARDS). Its use has not been validated in neonatal models.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We compared an in vivo lung ultrasound score against clinical and histological markers of acute lung injury, in a neonatal animal model, hypothesizing that POCUS would sensitively diagnose early acute lung injury in neonates and discern its severity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifteen anesthetized, ventilated 3\u2010day\u2010old neonatal piglets were divided into controls, moderate lung injury, or severe lung injury by graded treatment with oleic acid. Degree of lung injury was quantified at baseline, immediately after oleic acid administration, and 1\u2009hour after the evolution of acute lung injury, by blood gases, ventilation parameters and calculated oxygenation deficit; hemodynamic indices by echocardiography, and lung ultrasound obtained in an 8\u2010region grid of anterior and posterior zones, semi\u2010quantitatively analyzed by a blinded observer. Lungs were inflation\u2010fixed postmortem at last mean airway pressure, for histological assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Acute lung injury manifested in oleic acid\u2010treated groups as dose\u2010dependent capillary leak causing intravascular depletion and cardiac failure, hypoxemia with increasing intrapulmonary shunt fraction, decreased lung compliance, and resistance. Ultrasound scores of anterior regions distinguished moderate from severe injury; scores in posterior regions reached maximum values immediately after lung injury. POCUS score correlated with calculated intrapulmonary shunt fraction (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u2009.65) and with histological injury score (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u2009.61),<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.01.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that POCUS may be valuable in neonates for early quantification of acute lung injury or ARDS; and that nondependent ultrasound regions clearly distinguish severity of pulmonary edema.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.24993","type":"journal-article","created":{"date-parts":[[2020,8,2]],"date-time":"2020-08-02T08:20:32Z","timestamp":1596356432000},"page":"2913-2923","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Lung ultrasound predicts histological lung injury in a neonatal model of acute respiratory distress syndrome"],"prefix":"10.1002","volume":"55","author":[{"given":"Yasser N.","family":"Elsayed","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Section of Neonatology University of Manitoba Winnipeg Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha","family":"Hinton","sequence":"additional","affiliation":[{"name":"Biology of Breathing Theme Children's Hospital Research Institute of Manitoba Winnipeg Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Anesthesia University of Manitoba Winnipeg Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7279-5226","authenticated-orcid":false,"given":"Shyamala","family":"Dakshinamurti","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Section of Neonatology University of Manitoba Winnipeg Canada"},{"name":"Biology of Breathing Theme Children's Hospital Research Institute of Manitoba Winnipeg Canada"},{"name":"Department of Physiology University of Manitoba Winnipeg Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-020-2787-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.05015-11"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/2045894018766737"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00010.2008"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02533957"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-64"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12630-011-9517-3"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2016.05.005"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3182186d09"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(17)30214-X"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(17)30381-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(17)30380-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.07.76"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.24248"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1676975"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/fn.85.2.F127"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0210ST"},{"issue":"1","key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.54846\/jshap\/922","article-title":"An investigation of iron deficiency and anemia in piglets and the effect of iron status at weaning on post\u2010weaning performance","volume":"24","author":"Perri AM","year":"2016","journal-title":"J Swine Health Prod"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10739680701647410"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.149.3.7509706"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc6771"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2017.06.56"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000438453"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.1.9906091"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199804000-00020"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.02.00255102"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199103000-00021"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/2045894019841987"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001340050436"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199004000-00014"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-016-4328-1"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000287525.03140.3F"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2013.08.053"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2015.1797"},{"key":"e_1_2_8_36_1","article-title":"Prone versus supine position for lung ultrasound in neonates with respiratory distress [published online ahead of print September 3, 2019]","author":"Louis D","year":"2019","journal-title":"Am J Perinatol"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-018-2105-y"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40635-019-0241-6"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crad.2020.05.001"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2019.08.008"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2012.03.001"}],"container-title":["Pediatric Pulmonology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fppul.24993","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ppul.24993","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ppul.24993","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ppul.24993","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T05:52:34Z","timestamp":1723355554000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ppul.24993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,10]]},"references-count":40,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1002\/ppul.24993"],"URL":"https:\/\/doi.org\/10.1002\/ppul.24993","archive":["Portico"],"relation":{},"ISSN":["8755-6863","1099-0496"],"issn-type":[{"value":"8755-6863","type":"print"},{"value":"1099-0496","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,10]]},"assertion":[{"value":"2020-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}