{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T07:02:57Z","timestamp":1763535777473,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T00:00:00Z","timestamp":1603929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Bronchiolitis obliterans is a fatal respiratory disease characterized by the obliteration of small airways. Mesenchymal stem cells (MSCs) is a promising candidate for cell\u2010based therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the therapeutic effect of human umbilical cord\u2010derived mesenchymal stem cells (HUC\u2010MSCs) on a murine model of bronchiolitis obliterans like injury (BOLI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The murine model of BOLI was established by administrating of diacetyl (DA) via intratracheal instillation. Treatment of HUC\u2010MSCs or HUC\u2010MSCs culture medium (HUC\u2010MSCs\u2010CM) was conducted in the BOLI model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The pathogenic manifestations, lung function, and the number of neutrophils were similar between the oropharyngeal inhalation DA group (OPI\u2010DA), intratracheal instillation group (ITI\u2010DA); however, less reduction of weight and higher survival rate were observed in ITI\u2010DA groups. Compared with the control groups, the trend of weight loss was significantly reduced (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), and the pulmonary function was significantly improved (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) in HUC\u2010MSCs and HUC\u2010MSCs\u2010CM groups. Masson staining and hematoxylin and eosin staining showed that the deposition of collagen around bronchioles and blood vessels is less and airway epithelial cells and basal cells in lung tissue repaired better in HUC\u2010MSCs and HUC\u2010MSCs\u2010CM groups compared with the control groups. Immunofluorescence shows the expression of E\u2010cadherin and cytokeratin 5 (CK\u20105) were significantly higher in HUC\u2010MSCs and HUC\u2010MSCs\u2010CM groups compared with control groups, while HUC\u2010MSCs themselves did not express E\u2010cadherin or CK\u20105. The DiI label showed HUC\u2010MSCs gradually reduced after 2 days in the bronchus and 4 days in bronchiole.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HUC\u2010MSCs could help to repair airway epithelial cells in a murine model of BOLI. It might be related to paracrine factors of HUC\u2010MSCs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.25128","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T12:47:16Z","timestamp":1603284436000},"page":"129-137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of human umbilical cord\u2010derived mesenchymal stem cells on murine model of bronchiolitis obliterans like injury"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1920-9317","authenticated-orcid":false,"given":"Jilei","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Respiratory Disease, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders Ministry of Education Key Laboratory of Child Development and Disorders  Chongqing China"},{"name":"Chongqing Key Laboratory of Pediatrics  Chongqing 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