{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T19:44:07Z","timestamp":1780602247852,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:00:00Z","timestamp":1619395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nasal nitric oxide (nNO) measurement is recommended to screen for Primary Ciliary Dyskinesia (PCD) in subjects with suggestive history and symptoms. Clinical use of alternative methods (i.e., breath\u2010hold [BH], tidal breathing [TB]) in children unable to perform the gold standard slow Exhalation against a Resistance (ER) method has not been sufficiently evaluated.\u00a0We extracted retrospectively (2013\u20132019) 454 files (374 subjects) containing nNO results. Median [IQR] age at inclusion was 7.0 [4.7\u201311.0] years, 105 (28.1%) children were younger than 5\u2009years. ER or BH methods were more frequently mastered by children older than 5\u2009years compared to younger children (69.4% and 52.7% vs. 21% and 5.6%, respectively; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.0001), the latter succeeding only in TB measurement in 77.4% of cases.\u00a0In 130 files with both ER and BH measurements (nNO\u2010ER and nNO\u2010BH), nNO\u2010BH was 102 [96.2; 108.3]% that of nNO\u2010ER. In 175 files including nNO\u2010ER and nNO\u2010TB measurements, nNO\u2010TB was 64.4 [IQR: 53.7; 80.4]% that of nNO\u2010ER with an excellent correlation between nNO values (<jats:italic>r<\/jats:italic>\u2009=\u2009.94 [95% CI 0.91; 0.95]; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.0001) and discordance in the interpretation of nNO results in 16 (10.2%) cases. Final PCD diagnosis was similar in patients included before or after 5\u2009years of age (confirmed 16 (15.2%) and 48 (17.8%); excluded 81 (77.1%) and 192 (71.4%), respectively; <jats:italic>p<\/jats:italic>\u2009=\u2009.32).\u00a0In conclusion, reliable nNO\u2010BH and nNO\u2010ER results are interchangeable. Children tested with ER or with TB method have similar final PCD diagnosis. Alternative methods to measure nNO might be studied further for use in clinical practice.<\/jats:p>","DOI":"10.1002\/ppul.25432","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T21:14:15Z","timestamp":1618607655000},"page":"2242-2249","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Breath\u2010holding and tidal breathing nasal NO to screen children for Primary Ciliary Dyskinesia"],"prefix":"10.1002","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6417-382X","authenticated-orcid":false,"given":"Nicole","family":"Beydon","sequence":"first","affiliation":[{"name":"APHP, Unit\u00e9 Fonctionnelle de Physiologie\u2010Explorations Fonctionnelles Respiratoires (EFR) H\u00f4pital Armand\u2010Trousseau  Paris France"},{"name":"INSERM U934 Centre de Recherche Saint Antoine  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Tamalet","sequence":"additional","affiliation":[{"name":"AP\u2010HP, Centre de R\u00e9f\u00e9rence des Maladies Respiratoires Rares Service de pneumologie p\u00e9diatrique, H\u00f4pital Armand\u2010Trousseau  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Escudier","sequence":"additional","affiliation":[{"name":"AP\u2010HP D\u00e9partement de G\u00e9n\u00e9tique m\u00e9dicale Sorbonne Universit\u00e9, Inserm UMR_S933 Maladies g\u00e9n\u00e9tiques d'expression p\u00e9diatrique, H\u00f4pital Armand Trousseau  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Legendre","sequence":"additional","affiliation":[{"name":"AP\u2010HP D\u00e9partement de G\u00e9n\u00e9tique m\u00e9dicale Sorbonne Universit\u00e9, Inserm UMR_S933 Maladies g\u00e9n\u00e9tiques d'expression p\u00e9diatrique, H\u00f4pital Armand Trousseau  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Thouvenin","sequence":"additional","affiliation":[{"name":"INSERM U934 Centre de Recherche Saint Antoine  Paris France"},{"name":"AP\u2010HP, Centre de R\u00e9f\u00e9rence des Maladies Respiratoires Rares Service de pneumologie p\u00e9diatrique, H\u00f4pital Armand\u2010Trousseau  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01090-2016"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201805-0819ST"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202003-0835LE"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201311-2047OC"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201904-347OT"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0495-370"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.05.00015205"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00032610"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2005.086702"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2015.07.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02503-2017"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.1999.13f28.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.7.586"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00305503"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.126.4.1054"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2011.03.007"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23167"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00749-2015"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201305-110OC"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2015-208056"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200303-365OC"},{"key":"e_1_2_7_23_1","first-page":"CR80","article-title":"Nasal nitric oxide assessment in primary ciliary dyskinesia using aspiration, exhalation, and humming","volume":"14","author":"Santamaria F","year":"2008","journal-title":"Med Sci Monit Int Med J Exp Clin Res"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2008.02064.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3038.2010.00989.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","unstructured":"ATS\/ERS. 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