{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T09:49:14Z","timestamp":1756460954218,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T00:00:00Z","timestamp":1684886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hispanic people with cystic fibrosis (CF) have decreased life expectancy and earlier acquisition of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> compared to non\u2010Hispanic white individuals with CF. Racial and ethnic differences in the airway microbiome of CF may contribute to known health disparity, but have not been studied. The objective was to describe differences in the upper airway microbial community in Hispanic and non\u2010Hispanic white children with CF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This prospective, observational cohort study of 59\u00a0Hispanic and non\u2010Hispanic white children with CF, ages 2\u221210 years old, was performed at Texas Children's Hospital (TCH) from February 2019 to January 2020. Oropharyngeal swabs were collected from the cohort during clinic visit. Swab samples underwent sequencing (16S V4 rRNA), diversity analysis, and taxonomic profiling. Key demographic and clinical data were collected from the electronic medical record and the CF Foundation Patient Registry (CFFPR). Statistical analysis compared sequencing, demographic, and clinical data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found no significant difference in Shannon diversity or relative abundance of bacterial phyla between Hispanic and non\u2010Hispanic children with CF. However, a low abundant taxa\u2010 \u201cuncultured bacterium\u201d belonging to the order <jats:italic>Saccharimonadales<\/jats:italic> was significantly higher in Hispanic children (mean relative abundance\u2009=\u20090.13%) compared to the non\u2010Hispanic children (0.03%). Hispanic children had increased incidence of <jats:italic>P. aeruginosa<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20090.045) compared to non\u2010Hispanic children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We did not find a significant difference in the airway microbial diversity between Hispanic and non\u2010Hispanic white children with CF. However, we found a greater relative abundance of <jats:italic>Saccharimonadales<\/jats:italic> and higher incidence of <jats:italic>P. aeruginosa<\/jats:italic> in Hispanic children with CF.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ppul.26484","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T11:26:36Z","timestamp":1684927596000},"page":"2298-2307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The upper airway microbiome in Hispanic children with cystic fibrosis"],"prefix":"10.1002","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0074-6967","authenticated-orcid":false,"given":"John","family":"Palla","sequence":"first","affiliation":[{"name":"Division of Pulmonary and Sleep Medicine Ann &amp; Robert H. Lurie Children's Hospital Chicago Illinois USA"},{"name":"Department of Pediatrics Northwestern University Feinberg School of Medicine Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4534-1349","authenticated-orcid":false,"given":"Santosh","family":"Thapa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Texas Children's Microbiome Center Texas Children's Hospital Houston Texas USA"},{"name":"Department of Pathology and Immunology Baylor College of Medicine Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alamelu","family":"Venkatachalam","sequence":"additional","affiliation":[{"name":"Department of Pathology, Texas Children's Microbiome Center Texas Children's Hospital Houston Texas USA"},{"name":"Department of Pathology and Immunology Baylor College of Medicine Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica K.","family":"Runge","sequence":"additional","affiliation":[{"name":"Department of Pathology, Texas Children's Microbiome Center Texas Children's Hospital Houston Texas USA"},{"name":"Department of Pathology and Immunology Baylor College of Medicine Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4255-0310","authenticated-orcid":false,"given":"Theresa A.","family":"Laguna","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Sleep Medicine Ann &amp; Robert H. Lurie Children's Hospital Chicago Illinois USA"},{"name":"Department of Pediatrics Northwestern University Feinberg School of Medicine Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth Ann","family":"Luna","sequence":"additional","affiliation":[{"name":"Department of Pathology, Texas Children's Microbiome Center Texas Children's Hospital Houston Texas USA"},{"name":"Department of Pathology and Immunology Baylor College of Medicine Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23884"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00576-6"},{"key":"e_1_2_9_4_1","unstructured":"RegistryCFFP. 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