{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T10:48:54Z","timestamp":1775645334341,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      AUST\u201003 (ST242) has been reported to cause epidemics in people with CF (pwCF) from Australia and has been associated with multidrug resistance and increased morbidity and mortality. Here, we report an epidemic\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      (AUST\u201003) strain in South African pwCF detected at a public hospital and characterize the genomic antibiotic resistance determinants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      AUST\u201003 (ST242) study isolates were analysed with whole genome sequencing using the Illumina NextSeq2000 platform. Raw sequencing reads were processed using the Jekesa pipeline and multilocus sequence typing and genomic antibiotic resistance characterization was performed using public databases. Genetic relatedness between the study isolates and global\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      ST242 from public databases was determined using a maximum\u2010likelihood phylogenetic tree. Antibiotic susceptibility testing was performed using the disk diffusion and broth microdilution techniques.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 11\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      AUST\u201003 isolates were isolated from two children with CF. The majority (8\/11) of these isolates were multidrug\u2010resistant (MDR) or extensively drug resistant; and the multidrug efflux pumps MexAB\u2010OprM, MexCD\u2010OprJ, MexEF\u2010OprN, and MexXY\u2010OprM were the most clinically relevant antibiotic resistance determinants and were detected in all of the isolates. The study isolates were the most closely related to a 2020\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      AUST\u201003 (ST242) CF isolate from Russia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Epidemic MDR\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      strains are present at South African public CF clinics and need to be considered when implementing segregation and infection control strategies to prevent possible spread and outbreaks.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ppul.27202","type":"journal-article","created":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T09:10:38Z","timestamp":1723021838000},"page":"3340-3348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Detection of the epidemic\n                    <i>Pseudomonas aeruginosa<\/i>\n                    AUST\u201003 (ST242) strain in people with cystic fibrosis in South Africa"],"prefix":"10.1002","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1792-7975","authenticated-orcid":false,"given":"Thabo","family":"Hamiwe","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Health Sciences University of Pretoria Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0943-5970","authenticated-orcid":false,"given":"Debbie 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