{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T16:20:07Z","timestamp":1778170807040,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Pulmonology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Uncontrolled or severe asthma results in symptomatic usage of short\u2010acting \u00df2\u2010agonist (SABA) usage. MicroRNAs (miRNAs) are posttranslational regulators that can influence asthma biology. This study aims to identify miRNAs that are associated with increased SABA usage.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Small RNA sequenced from blood serum of 1,132 children with asthma aged 6 to 14 years in the Genetics of Asthma in Costa Rica Study was used for this analysis. Logistic regression identified miRNAs in patients who required increased SABA usage. These miRNA were validated for association with SABA\u2010induced bronchodilator responsiveness (BDR). Gene target pathway analysis was performed on validated miRNAs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010one miRNAs were significantly associated with increased SABA usage with OR ranging from 0.87 to 1.23. Two miRNAs, miR\u2010378a\u20103p and miR\u2010144\u20103p, had odds ratio 1.14 (1\u20131.29,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.05) and 1.11 (1.01\u20131.22,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.035), respectively, for increased SABA usage and were also significantly associated with bronchodilator response. Furthermore, a linear regression analysis involving these miRNA and bronchodilator response revealed that increased miR\u2010378a\u20103p correlated with decreased BDR and increased expression of miR\u2010144\u20103p correlated with improving pulmonary function with bronchodilators. In gene target Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the erythroblastosis viral oncogene signaling pathway had among one of the highest fold enrichment and\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Increased expression of miR\u2010378a\u20103p and miR\u2010144\u20103p was seen in this patient population who required increased SABA usage. There were different bronchodilatory effects seen in these two miRNAs, suggesting different potential mechanisms underlying increased SABA usage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ppul.27236","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T07:49:18Z","timestamp":1724831358000},"page":"3491-3498","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification of MicroRNAs in children with increased asthma bronchodilator usage in genetics of asthma in Costa Rica Study"],"prefix":"10.1002","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3776-9027","authenticated-orcid":false,"given":"Richard","family":"Wong","sequence":"first","affiliation":[{"name":"Division of Pediatric Respiratory Medicine, Rady Children's Hospital University of California San Diego San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brinda","family":"Desai","sequence":"additional","affiliation":[{"name":"Division of Pulmonology and Critical Care University of California San Diego San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rinku","family":"Sharma","sequence":"additional","affiliation":[{"name":"Channing Division of Network Medicine Brigham and Women's Hospital Boston Massachusetts USA"},{"name":"Harvard Medical School Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Upasna","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Division of Neurology Yale University New Haven Connecticut USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvin T.","family":"Kho","sequence":"additional","affiliation":[{"name":"Channing Division of Network Medicine Brigham and Women's Hospital Boston Massachusetts USA"},{"name":"Harvard Medical School Boston Massachusetts USA"},{"name":"Computational Health Informatics Program Boston Children's Hospital Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott T.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Channing Division of Network Medicine Brigham and Women's Hospital Boston Massachusetts USA"},{"name":"Harvard Medical School Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6139-5320","authenticated-orcid":false,"given":"Juan C.","family":"Celed\u00f3n","sequence":"additional","affiliation":[{"name":"Division of Pediatric Pulmonary Medicine, UPMC Children's Hospital of Pittsburgh University of Pittsburgh Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"McGeachie","sequence":"additional","affiliation":[{"name":"Channing Division of Network Medicine Brigham and Women's Hospital Boston Massachusetts USA"},{"name":"Harvard Medical School Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelan","family":"Tantisira","sequence":"additional","affiliation":[{"name":"Division of Pediatric Respiratory Medicine, Rady Children's Hospital University of California San Diego San Diego California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30925-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2678"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(23)00230-8"},{"key":"e_1_2_9_5_1","unstructured":"2020Focused Updates to the Asthma Management Guidelines: A Report from the National Asthma Education and Prevention Program Coordinating Committee Expert Panel Working 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